Fate of swallowed interleukin 8 and TNFα, and effect on the Wistar rat gut

Briars, G. L., Greer, R. M., Cleghorn, G. J., & Shepherd, R. W. (2002) Fate of swallowed interleukin 8 and TNFα, and effect on the Wistar rat gut. Journal of Cystic Fibrosis, 1(2), pp. 94-98.

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To evaluate the passage of cytokines through the gastrointestinal tract, we investigated the digestion of interleukin-8 (IL-8) and tumour necrosis factor α (TNFα), in vitro and in vivo, and their propensity to induce intestinal inflammation. We serially immuno-assayed IL-8 and TNFα solutions co-incubated with each of three pancreatin preparations at pH 4.5 and pH 8. We gavaged IL-8, TNFα and marker into 15 Wistar rats, and measured their faecal cytokine concentrations by ELISA and histologically examined their guts. IL-8 immunoreactivity was extinguished by all pancreatin preparations after 1 h of incubation at 37 °C. TNFα concentration progressively fell from 1 to 4 h with all enzyme preparations. Buffer control samples maintained their cytokine concentrations throughout incubation. No IL-8 or TNFα was detected in any rat faecal pellets. There was no significant proinflammatory effect of the gavaged cytokines on rat intestine. IL-8 and TNFα in aqueous solution could well be fully digested in the CF gut when transit time is normal and exogenous enzymes are provided, although cytokines swallowed in viscous sputum may be protected from such digestion

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ID Code: 89600
Item Type: Journal Article
Refereed: Yes
Additional Information: Export Date: 1 September 2015
Correspondence Address: Briars, G.L.; Paediatric Gastroenterology, West Suffolk Hospital, Hardwick Lane, Bury St Edmunds, Suffolks IP33 2QZ, United Kingdom; email: g.briars@doctors.org.uk
Keywords: Interleukin-8, Pancreatic enzymes, Tumor necrosis factor α (TNFα), Wistar rats, cytokine, gastrointestinal agent, interleukin 8, marker, pancreatin, tumor necrosis factor, tumor necrosis factor alpha, animal, animal experiment, animal tissue, aqueous solution, article, chemistry, concentration (parameters), controlled study, digestion, drug effect, enteritis, enzymatic degradation, enzyme linked immunosorbent assay, feces, feces level, feeding, gastrointestinal tract, gastrointestinal transit, histopathology, human, immunoassay, immunology, immunoreactivity, in vitro study, in vivo study, incubation time, inflammation, intestine transit time, male, nonhuman, oral drug administration, pH, protection, rat, rat strain, sputum, temperature, viscosity, Administration, Oral, Animals, Cytokines, Gastrointestinal Agents, Humans, Rats, Rats, Wistar, Tumor Necrosis Factor-alpha
DOI: 10.1016/S1569-1993(02)00035-8
ISSN: 1569-1993
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > Schools > School of Exercise & Nutrition Sciences
Copyright Owner: © 2002 European Cystic Fibrosis Society
Deposited On: 28 Oct 2015 00:21
Last Modified: 28 Oct 2015 00:21

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