Cloning and gastrointestinal expression of rat hephaestin: Relationship to other iron transport proteins
Frazer, D. M., Vulpe, C. D., McKie, A. T., Wilkins, S. J., Trinder, D., Cleghorn, G. J., & Anderson, G. J. (2001) Cloning and gastrointestinal expression of rat hephaestin: Relationship to other iron transport proteins. American Journal of Physiology - Gastrointestinal and Liver Physiology, 281(4), G931-G939.
The membrane-bound ceruloplasmin homolog hephaestin plays a critical role in intestinal iron absorption. The aims of this study were to clone the rat hephaestin gene and to examine its expression in the gastrointestinal tract in relation to other genes encoding iron transport proteins. The rat hephaestin gene was isolated from intestinal mRNA and was found to encode a protein 96% identical to mouse hephaestin. Analysis by ribonuclease protection assay and Western blotting showed that hephaestin was expressed at high levels throughout the small intestine and colon. Immunofluorescence localized the hephaestin protein to the mature villus enterocytes with little or no expression in the crypts. Variations in iron status had a small but nonsignificant effect on hephaestin expression in the duodenum. The high sequence conservation between rat and mouse hephaestin is consistent with this protein playing a central role in intestinal iron absorption, although its precise function remains to be determined.
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|Item Type:||Journal Article|
|Additional Information:||Cited By :105
Export Date: 1 September 2015
Correspondence Address: Anderson, G.J.; Clinical Sciences Unit, Queensland Inst. of Medical Research, Post Office Royal Brisbane Hospital, Brisbane, QLD 4029, Australia; email: gregA@qimr.edu.au
|Keywords:||Absorption, Divalent metal transporter-1, Intestine, Ireg1, carrier protein, complementary DNA, iron, messenger RNA, natural resistance associated macrophage protein 2, regulator protein, synthetic peptide, unclassified drug, animal tissue, article, duodenum, immunoblotting, immunofluorescence, intestine absorption, intestine cell, iron absorption, iron responsive element, iron transport, male, molecular cloning, nonhuman, nucleotide sequence, polymerase chain reaction, priority journal, protein analysis, rat, Amino Acid Sequence, Animals, Carrier Proteins, Cation Transport Proteins, Cloning, Molecular, Digestive System, Histocompatibility Antigens Class I, HLA Antigens, Humans, Immunohistochemistry, Iron-Binding Proteins, Membrane Proteins, Mice, Molecular Sequence Data, Rats, Rats, Sprague-Dawley, Receptors, Transferrin, RNA, Messenger, Sequence Alignment, Tissue Distribution|
|Divisions:||Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > Schools > School of Exercise & Nutrition Sciences
|Deposited On:||27 Oct 2015 23:15|
|Last Modified:||27 Oct 2015 23:17|
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