Studies of water and electrolyte movement from oral rehydration solutions (rice- and glucose-based) across a normal and secreting gut using a dual isotope tracer technique in a rat perfusion model

Wall, C.R., Bates, M.S., Cleghorn, G.J., & Ward, L. (1997) Studies of water and electrolyte movement from oral rehydration solutions (rice- and glucose-based) across a normal and secreting gut using a dual isotope tracer technique in a rat perfusion model. Alimentary Pharmacology and Therapeutics, 11(3), pp. 581-587.

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Abstract

Aims: To establish a model to measure bidirectional flow of water from a glucose oral rehydration solution (G-ORS) and a newly developed rice-based oral rehydration solution (R-ORS) using a dual isotope tracer technique in a rat perfusion model. To measure net water, sodium and potassium absorption from the ORS.

Methods: In viva steady-state perfusion studies were carried out in normal and secreting (induced by cholera toxin) rat small intestine (n = 11 in each group). To determine bidirectional flow of water from the ORS the animals were initially labelled with tritium, and deuterium was added to the perfusion solution. Sequential perfusate and blood samples were collected after attainment of steady-state conditions and analysed for water and electrolyte content.

Results: There was a significant increase in net water absorption from the R-ORS compared to the G-ORS in both the normal (P < 0.02) and secreting intestine (P < 0.05). Water efflux was significantly reduced in the R-ORS group compared to the G-ORS group in both the normal (P < 0.01) and the secreting intestine (P < 0.01). There was an increase in sodium absorption in the R-ORS group compared to the G-ORS. The G-ORS produced a significantly greater blood glucose level at 75 min compared to the R-ORS (P < 0.03) in the secreting intestine.

Conclusions: This study demonstrates the improved water absorption from a rice-based ORS in both the normal and secreting intestine. Evidence that the absorption of water may be influenced by the osmolality of the ORS was also demonstrated.

Impact and interest:

6 citations in Scopus
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5 citations in Web of Science®

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ID Code: 89615
Item Type: Journal Article
Refereed: Yes
Keywords: electrolyte, glucose, oral rehydration solution, water, animal experiment, animal model, article, cholera, dehydration, diarrhea, drug formulation, gastroenteritis, male, nonhuman, oral drug administration, oral rehydration therapy, priority journal, rat, rice, sodium absorption, water flow, Absorption, Animals, Blood Glucose, Deuterium, Electrolytes, Fluid Therapy, Potassium, Rats, Rats, Wistar, Rehydration Solutions, Sodium, Tritium
DOI: 10.1046/j.1365-2036.1997.00171.x
ISSN: 0269-2813
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 1997 Blackwell Science Ltd.
Deposited On: 28 Oct 2015 02:23
Last Modified: 28 Oct 2015 04:26

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