Heterogeneity of miR-10b expression in circulating tumor cells

Gasch, Christin, Plummer, Prue N., Jovanovic, Lidija, McInnes, Linda M., Wescott, David, Saunders, Christobel M., Schneeweiss, Andreas, Wallwiener, Markus, Nelson, Colleen, Spring, Kevin J., Riethdorf, Sabine, Thompson, Erik W., Pantel, Klaus, & Mellick, Albert S. (2015) Heterogeneity of miR-10b expression in circulating tumor cells. Scientific Reports, 5, Article number: 15980.

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Abstract

Circulating tumor cells (CTCs) in the blood of cancer patients are recognized as important potential targets for future anticancer therapies. As mediators of metastatic spread, CTCs are also promising to be used as € liquid biopsyto aid clinical decision-making. Recent work has revealed potentially important genotypic and phenotypic heterogeneity within CTC populations, even within the same patient. MicroRNAs (miRNAs) are key regulators of gene expression and have emerged as potentially important diagnostic markers and targets for anti-cancer therapy. Here, we describe a robust in situ hybridization (ISH) protocol, incorporating the CellSearch ® CTC detection system, enabling clinical investigation of important miRNAs, such as miR-10b on a cell by cell basis. We also use this method to demonstrate heterogeneity of such as miR-10b on a cell-by-cell basis. We also use this method to demonstrate heterogeneity of miR-10b in individual CTCs from breast, prostate and colorectal cancer patients.

Impact and interest:

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ID Code: 89637
Item Type: Journal Article
Refereed: Yes
Keywords: Prostate cancer, Cancer genetics, Circulating tumour cells, miR-10b, gene expression
DOI: 10.1038/srep15980
ISSN: 2045-2322
Subjects: Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000) > MEDICAL BIOTECHNOLOGY (100400) > Medical Biotechnology Diagnostics (incl. Biosensors) (100402)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Cell Biology (111201)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Genetics (111203)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Funding:
Copyright Statement: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Deposited On: 28 Oct 2015 22:31
Last Modified: 29 Jul 2016 04:41

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