Structural determination of the K14 capsular polysaccharide from an ST25 Acinetobacter baumannii isolate, D46

Kenyon, Johanna J., Hall, Ruth M., & De Castro, Cristina (2015) Structural determination of the K14 capsular polysaccharide from an ST25 Acinetobacter baumannii isolate, D46. Carbohydrate Research, 417, pp. 52-56.

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Abstract

The structure of the capsular polysaccharide (CPS) recovered from D46, an extensively antibiotic resistant ST25 Acinetobacter baumannii clinical isolate, was elucidated. The structure was resolved on the basis of NMR spectroscopy and chemical analyses, and was found to contain a branched neutral pentasaccharide with a backbone composed of GalpNAc and Galp residues, all d configured, and a d-Glcp side group. The KL14 gene cluster found in the D46 genome includes genes for four glycosyltransferases but no modules for synthesis of complex sugars, and this is consistent with the structure of K14. The K14 structure and KL14 sequence clarify the relationship between the structure and K locus sequence for A. nosocomialis isolate LUH5541. The identity of the first sugar of the K14 repeat unit (K unit), and the functions of the four encoded glycosyltransferases and Wzy polymerase were predicted.

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ID Code: 90596
Item Type: Journal Article
Refereed: Yes
Keywords: Acinetobacter baumannii, Capsular polysaccharide, Capsule, K locus, KL14 gene cluster
DOI: 10.1016/j.carres.2015.09.002
ISSN: 0008-6215
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Biochemistry and Cell Biology not elsewhere classified (060199)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Bacteriology (060501)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Microbial Genetics (060503)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2015 Elsevier Ltd.
Deposited On: 23 Nov 2015 23:52
Last Modified: 25 Nov 2015 02:14

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