A potential epigenetic marker mediating serum folate and vitamin B12 levels contributes to ischemic stroke risk
Loo, Keat Wei, Griffiths, Lyn R., Sutherland, Heidi, Haupt, Larisa M., & Bhaskar, Shalini (2015) A potential epigenetic marker mediating serum folate and vitamin B12 levels contributes to ischemic stroke risk. BioMed Research International, 2015(167976).
Background and Purpose
Stroke is a multifactorial disease that may be associated with aberrant DNA methylation profiles.We investigated epigenetic dysregulation for the MTHFR gene among ischaemic stroke patients.
Cases (n=297) and controls (n=110) were recruited after obtaining signed written informed consent, following a screening process against the inclusion/exclusion criteria. Serum vitamin metabolites (folate, vitamin B12 and homocysteine) were determined using immunoassays and methylation profiles for CpGs A and B in the MTHFR gene were determined using bisulfitepyrosequencing method.
Methylation of MTHFR significantly increased the susceptibility risk for ischemic stroke. In particular, CpG A outperformed CpG B in mediating folate and vitamin B12 levels to increase ischemic stroke susceptibility risks by 4.73 fold. CpGs A and B were not associated with either serum homocysteine levels or ischemic stroke severity.
CpG A is a potential epigenetic marker in mediating serum folate and vitamin B12 to contribute to ischemic stroke.
Impact and interest:
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|Item Type:||Journal Article|
|Keywords:||Ischemic Stroke, Epigenetics, Serum Folate, Vitamin B12|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400) > Epigenetics (incl. Genome Methylation and Epigenomics) (060404)
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Owner:||Copyright 2015 Loo KeatWei et al.|
|Copyright Statement:||This is an open access article distributed under the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
|Deposited On:||25 Nov 2015 02:19|
|Last Modified:||29 Nov 2015 01:49|
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