A potential epigenetic marker mediating serum folate and vitamin B12 levels contributes to ischemic stroke risk

Loo, Keat Wei, Griffiths, Lyn R., Sutherland, Heidi, Haupt, Larisa M., & Bhaskar, Shalini (2015) A potential epigenetic marker mediating serum folate and vitamin B12 levels contributes to ischemic stroke risk. BioMed Research International, 2015(167976).

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Abstract

Background and Purpose

Stroke is a multifactorial disease that may be associated with aberrant DNA methylation profiles.We investigated epigenetic dysregulation for the MTHFR gene among ischaemic stroke patients.

Methods

Cases (n=297) and controls (n=110) were recruited after obtaining signed written informed consent, following a screening process against the inclusion/exclusion criteria. Serum vitamin metabolites (folate, vitamin B12 and homocysteine) were determined using immunoassays and methylation profiles for CpGs A and B in the MTHFR gene were determined using bisulfitepyrosequencing method.

Results

Methylation of MTHFR significantly increased the susceptibility risk for ischemic stroke. In particular, CpG A outperformed CpG B in mediating folate and vitamin B12 levels to increase ischemic stroke susceptibility risks by 4.73 fold. CpGs A and B were not associated with either serum homocysteine levels or ischemic stroke severity.

Conclusion

CpG A is a potential epigenetic marker in mediating serum folate and vitamin B12 to contribute to ischemic stroke.

Impact and interest:

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ID Code: 90714
Item Type: Journal Article
Refereed: Yes
Keywords: Ischemic Stroke, Epigenetics, Serum Folate, Vitamin B12
DOI: 10.1155/2015/167976
ISSN: 2314-6133
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400) > Epigenetics (incl. Genome Methylation and Epigenomics) (060404)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2015 Loo KeatWei et al.
Copyright Statement: This is an open access article distributed under the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Deposited On: 25 Nov 2015 02:19
Last Modified: 29 Nov 2015 01:49

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