Rapid isolation and detection of erythropoietin in blood plasma by magnetic core gold nanoparticles and portable Raman spectroscopy
Agoston, Roland, Izake, Emad L., Sivanesan, Arumugam, Lott, William B., Sillence, Martin, & Steel, Rohan (2016) Rapid isolation and detection of erythropoietin in blood plasma by magnetic core gold nanoparticles and portable Raman spectroscopy. Nanomedicine: Nanotechnology, Biology and Medicine, 12(3), pp. 633-641.
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Isolating, purifying, and identifying proteins in complex biological matrices is often difficult, time consuming, and unreliable. Herein we describe a rapid screening technique for proteins in biological matrices that combines selective protein isolation with direct surface enhanced Raman spectroscopy (SERS) detection. Magnetic core gold nanoparticles were synthesised, characterised, and subsequently functionalized with recombinant human erythropoietin (rHuEPO)-specific antibody. The functionalized nanoparticles were used to capture rHuEPO from horse blood plasma within 15 minutes. The selective binding between the protein and the functionalized nanoparticles was monitored by SERS. The purified protein was then released from the nanoparticles’ surface and directly spectroscopically identified on a commercial nanopillar SERS substrate. ELISA independently confirmed the SERS identification and quantified the released rHuEPO. Finally, the direct SERS detection of the extracted protein was successfully demonstrated for in-field screening by a handheld Raman spectrometer within 1 minute sample measurement time.
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|Item Type:||Journal Article|
|Keywords:||selective isolation, label-free SERS, magnetic core gold nanoparticles, erythropoietin, in-field screening, proteins in biological fluids|
|Subjects:||Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000)
Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > ANALYTICAL CHEMISTRY (030100)
Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000) > NANOTECHNOLOGY (100700)
|Divisions:||Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Schools > School of Earth, Environmental & Biological Sciences
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Facilities:||Science and Engineering Centre, Central Analytical Research Facility|
|Copyright Owner:||Copyright 2015 Elsevier Inc.|
|Copyright Statement:||This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/|
|Deposited On:||09 Dec 2015 06:06|
|Last Modified:||30 May 2016 05:03|
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