Packed bed bioreactor for the isolation and expansion of placental-derived Mesenchymal Stromal Cells
Osiecki, Michael J., Michl, Thomas D., Babur, Betul Kul, Kabiri, Mahboubeh, Atkinson, Kerry, Lott, William B., Griesser, Hans J., & Doran, Michael R. (2015) Packed bed bioreactor for the isolation and expansion of placental-derived Mesenchymal Stromal Cells. PLoS ONE, 10(12), e0144941.
Large numbers of Mesenchymal stem/stromal cells (MSCs) are required for clinical relevant doses to treat a number of diseases. To economically manufacture these MSCs, an automated bioreactor system will be required. Herein we describe the development of a scalable closed-system, packed bed bioreactor suitable for large-scale MSCs expansion. The packed bed was formed from fused polystyrene pellets that were air plasma treated to endow them with a surface chemistry similar to traditional tissue culture plastic. The packed bed was encased within a gas permeable shell to decouple the medium nutrient supply and gas exchange. This enabled a significant reduction in medium flow rates, thus reducing shear and even facilitating single pass medium exchange. The system was optimised in a small-scale bioreactor format (160 cm2) with murine-derived green fluorescent protein-expressing MSCs, and then scaled-up to a 2800 cm2 format. We demonstrated that placental derived MSCs could be isolated directly within the bioreactor and subsequently expanded. Our results demonstrate that the closed system large-scale packed bed bioreactor is an effective and scalable tool for large-scale isolation and expansion of MSCs.
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|Item Type:||Journal Article|
|Keywords:||mesenchymal stromal cells, packed bed bioreactor, cell expansion|
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Facilities:||Science and Engineering Centre, Central Analytical Research Facility|
|Copyright Owner:||Copyright 2015 Osiecki et al.|
|Copyright Statement:||This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited|
|Deposited On:||16 Dec 2015 06:37|
|Last Modified:||13 Nov 2016 23:18|
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