Packed bed bioreactor for the isolation and expansion of placental-derived Mesenchymal Stromal Cells

Osiecki, Michael J., Michl, Thomas D., Babur, Betul Kul, Kabiri, Mahboubeh, Atkinson, Kerry, Lott, William B., Griesser, Hans J., & Doran, Michael R. (2015) Packed bed bioreactor for the isolation and expansion of placental-derived Mesenchymal Stromal Cells. PLoS ONE, 10(12), e0144941.

View at publisher (open access)

Abstract

Large numbers of Mesenchymal stem/stromal cells (MSCs) are required for clinical relevant doses to treat a number of diseases. To economically manufacture these MSCs, an automated bioreactor system will be required. Herein we describe the development of a scalable closed-system, packed bed bioreactor suitable for large-scale MSCs expansion. The packed bed was formed from fused polystyrene pellets that were air plasma treated to endow them with a surface chemistry similar to traditional tissue culture plastic. The packed bed was encased within a gas permeable shell to decouple the medium nutrient supply and gas exchange. This enabled a significant reduction in medium flow rates, thus reducing shear and even facilitating single pass medium exchange. The system was optimised in a small-scale bioreactor format (160 cm2) with murine-derived green fluorescent protein-expressing MSCs, and then scaled-up to a 2800 cm2 format. We demonstrated that placental derived MSCs could be isolated directly within the bioreactor and subsequently expanded. Our results demonstrate that the closed system large-scale packed bed bioreactor is an effective and scalable tool for large-scale isolation and expansion of MSCs.

Impact and interest:

1 citations in Scopus
Search Google Scholar™
1 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

3 since deposited on 16 Dec 2015
3 in the past twelve months

Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 91288
Item Type: Journal Article
Refereed: Yes
Keywords: mesenchymal stromal cells, packed bed bioreactor, cell expansion
DOI: 10.1371/journal.pone.0144941
ISSN: 1932-6203
Divisions: Current > Schools > School of Biomedical Sciences
Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Funding:
  • WMI CRC/project 1.06
Facilities: Science and Engineering Centre, Central Analytical Research Facility
Copyright Owner: Copyright 2015 Osiecki et al.
Copyright Statement: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Deposited On: 16 Dec 2015 06:37
Last Modified: 13 Nov 2016 23:18

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page