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Developmental and hormonal regulation of direct shoot organogenesis and somatic embryogenesis in sugarcane (Saccharum spp. interspecific hybrids) leaf culture

Lakshmanan, Prakash, Geijskes, Robert J., Wang, Lifang, Elliott, Adrian, Grof, Chris P.L., Berding, Nils, & Smith, Grant R. (2006) Developmental and hormonal regulation of direct shoot organogenesis and somatic embryogenesis in sugarcane (Saccharum spp. interspecific hybrids) leaf culture. Plant Cell Reports, 25(10), pp. 1007-1015.

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Abstract

Rapid and efficient in vitro regeneration methods that minimise somaclonal variation are critical for the genetic transformation and mass propagation of commercial varieties. Using a transverse thin cell layer culture system, we have identified some of the developmental and physiological constraints that limit high-frequency regeneration in sugarcane leaf tissue. Tissue polarity and consequently the orientation of the explant in culture, size and developmental phase of explant, and auxin concentration play a significant role in determining the organogenic potential of leaf tissue in culture. Both adventitious shoot production and somatic embryogenesis occurred on the proximal cut surface of the explant, and a regeneration gradient, decreasing gradually from the basal to the distal end, exists in the leaf roll. Importantly, auxin, when added to the culture medium, reduced this spatial developmental constraint, as well as the effect of genotype on plant regeneration. Transverse sections (1–2 mm thick) ob- tained from young leaf spindle rolls and orienting explants with its distal end facing the medium (directly in contact with medium) are critical for maximum regeneration. Shoot regeneration was observed as early as 3 weeks on MS medium supplemented with α-naphthalenencetic acid (NAA) and 6-benzyladenine, while somatic embryogenesis or both adventitious shoot organogenesis and somatic embryogenesis occurred on medium with NAA and chlorophenoxyacetic acid. Twenty shoots or more could be generated from a single transverse section explant. These shoots regenerated roots and successfully established after transplanted to pots. Large numbers of plantlets can be regenerated directly and rapidly using this system. SmartSettr, the registered name for this process and the plants produced, will have significant practical applications for the mass propagation of new cultivars and in genetic modification programs. The SmartSettr system has already been used commercially to produce substantial numbers of plants of orange rust-resistant and new cultivars in Australia.

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31 citations in Scopus
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27 citations in Web of Science®

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ID Code: 9191
Item Type: Journal Article
Additional Information: For more information, please refer to the journal’s website (see link) or contact the author. Author contact details: j.geijskes@qut.edu.au
DOI: 10.1007/s00299-006-0154-1
ISSN: 1432-203X
Subjects: Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000) > AGRICULTURAL BIOTECHNOLOGY (100100) > Genetically Modified Field Crops and Pasture (100105)
Divisions: Current > Research Centres > Centre for Tropical Crops and Biocommodities
Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2006 Springer
Copyright Statement: The original publication is available at SpringerLink http://www.springerlink.com
Deposited On: 29 Aug 2007
Last Modified: 29 Feb 2012 23:30

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