KRAS variation and risk of endometriosis

Zhao, Z. Z., Nyholt, D.R., Le, L., Martin, N. G., James, M. R., Treloar, S. A., & Montgomery, G. W. (2006) KRAS variation and risk of endometriosis. Molecular Human Reproduction, 12(11), pp. 671-676.

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Endometriosis is a common gynaecological disease with symptoms of pelvic pain and infertility which affects 7-10% of women in their reproductive years. Activation of an oncogenic allele of Kirsten rat sarcoma viral oncogene homologue (KRAS) in the reproductive tract of mice resulted in the development of endometriosis. We hypothesized that variation in KRAS may influence risk of endometriosis in humans. Thirty tagSNPs spanning a region of 60.7 kb across the KRAS locus were genotyped using iPLEX chemistry on a MALDI-TOF MassARRAY platform in 959 endometriosis cases and 959 unrelated controls, and data were analysed for association with endometriosis. Genotypes were obtained for most individuals with a mean completion rate of 99.1%. We identified six haplotype blocks across the KRAS locus in our sample. There were no significant differences between cases and controls in the frequencies of individual single-nucleotide polymorphisms (SNPs) or haplotypes. We also developed a rapid method to screen for 11 common KRAS and BRAF mutations on the Sequenom MassARRAY system. The assay detected all mutations previously identified by direct sequencing in a panel of positive controls. No germline variants for KRAS or BRAF were detected. Our results demonstrate that any risk of endometriosis in women because of common variation in KRAS must be very small.

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31 citations in Web of Science®
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ID Code: 92151
Item Type: Journal Article
Refereed: Yes
Additional Information: Zhao, Zhen Zhen
Nyholt, Dale R
Le, Lien
Martin, Nicholas G
James, Michael R
Treloar, Susan A
Montgomery, Grant W
Research Support, Non-U.S. Gov't
2006/09/16 09:00
Mol Hum Reprod. 2006 Nov;12(11):671-6. Epub 2006 Sep 14.
Keywords: Adolescent, Adult, Aged, Aged, 80 and over, Case-Control Studies, Cell Line, Tumor, DNA Mutational Analysis, Endometriosis/epidemiology/*genetics/surgery, Female, *Genes, ras, Genetic Predisposition to Disease, Genotype, Haplotypes/genetics, Humans, Middle Aged, Polymorphism, Single Nucleotide, Proto-Oncogene Proteins B-raf/genetics, Proto-Oncogene Proteins p21(ras)/genetics/physiology, Queensland/epidemiology, Retrospective Studies, Risk, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
DOI: 10.1093/molehr/gal078
ISSN: 1360-9947
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: © The Author 2006
Deposited On: 22 Jan 2016 01:53
Last Modified: 07 Feb 2016 23:56

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