Enrichment and identification of glycoproteins in human saliva using lectin magnetic bead arrays
Caragata, Michael, Shah, Alok K., Schulz, Benjamin L., Hill, Michelle M., & Punyadeera, Chamindie (2016) Enrichment and identification of glycoproteins in human saliva using lectin magnetic bead arrays. Analytcial Biochemistry, 497, pp. 76-82.
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Aberrant glycosylation of proteins is a hallmark of tumorigenesis, and could provide diagnostic value in cancer detection. Human saliva is an ideal source of glycoproteins due to the relatively high proportion of glycosylated proteins in the salivary proteome. Moreover, saliva collection is non-invasive, technically straightforward and the sample collection and storage is relatively easy. Although, differential glycosylation of proteins can be indicative of disease states, identification of differential glycosylation from clinical samples is not trivial. To facilitate salivary glycoprotein biomarker discovery, we optimised a method for differential glycoprotein enrichment from human saliva based on lectin magnetic bead arrays (saLeMBA). Selected lectins from distinct reactivity groups were used in the saLeMBA platform to enrich salivary glycoproteins from healthy volunteer saliva. The technical reproducibility of saLeMBA was analysed with LC-MS/MS to identify the glycosylated proteins enriched by each lectin. Our saLeMBA platform enabled robust glycoprotein enrichment in a glycoprotein- and lectin-specific manner consistent with known protein-specific glycan profiles. We demonstrated that saLeMBA is a reliable method to enrich and detect glycoproteins present in human saliva.
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|Item Type:||Journal Article|
|Keywords:||Saliva proteomics, Biomarker discovery, Glycosylation, Lectins, Magnetic Beads|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Analytical Biochemistry (060101)|
|Divisions:||Current > Schools > School of Biomedical Sciences
Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Owner:||Copyright 2016 Elsevier|
|Copyright Statement:||Licensed under the Creative Commons Attribution; Non-Commercial; No-Derivatives 4.0 International. DOI: 10.1016/j.ab.2015.11.024|
|Deposited On:||03 Feb 2016 03:34|
|Last Modified:||07 Feb 2016 09:36|
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