Investigation of the effects of extracellular osmotic pressure on morphology and mechanical 1 properties of individual chondrocyte

Nguyen, Trung Dung, Oloyede, Adekunle, Singh, Sanjleena, & Gu, YuanTong (2016) Investigation of the effects of extracellular osmotic pressure on morphology and mechanical 1 properties of individual chondrocyte. Cell Biochemistry and Biophysics, 74(2), pp. 229-240.

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Abstract

It has been demonstrated that most cells of the body respond to osmotic pressure in a systematic manner. The disruption of the collagen network in the early stages of osteoarthritis causes an increase in water content of cartilage which leads to a reduction of pericellular osmolality in chondrocytes distributed within the extracellular environment. It is therefore arguable that an insight into the mechanical properties of chondrocytes under varying osmotic pressure would provide a better understanding of chondrocyte mechanotransduction and potentially contribute to knowledge on cartilage degeneration. In this present study, the chondrocyte cells were exposed to solutions with different osmolality. Changes in their dimensions and mechanical properties were measured over time. Atomic Force Microscopy (AFM) was used to apply load at various strain-rates and the force-time curves were logged. The thin-layer elastic model was used to extract the elastic stiffness of chondrocytes at different strain-rates and at different solution osmolality. In addition, the porohyperelastic (PHE) model was used to investigate the strain-rate dependent responses under the loading and osmotic pressure conditions. The results revealed that the hypo-osmotic external environment increased chondrocyte dimensions and reduced Young’s modulus of the cells at all strain-rates tested. In contrast, the hyper-osmotic external environment reduced dimensions and increased Young’s modulus. Moreover, by using the PHE model coupled with inverse FEA simulation, we established that the hydraulic permeability of chondrocytes increased with decreasing extracellular osmolality which is consistent with previous work in the literature. This could be due to a higher intracellular fluid volume fraction with lower osmolality.

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ID Code: 92633
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Cell biomechanics, osmotic pressure, AFM, strain-rate, mechanical properties
DOI: 10.1007/s12013-016-0721-1
ISSN: 1559-0283
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomechanical Engineering (090302)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > MECHANICAL ENGINEERING (091300) > Numerical Modelling and Mechanical Characterisation (091307)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Funding:
Facilities: Science and Engineering Centre, Central Analytical Research Facility
Copyright Owner: Copyright 2016 Springer
Copyright Statement: The final publication is available at Springer via http://dx.doi.org/10.1007/s12013-016-0721-1
Deposited On: 03 Feb 2016 23:41
Last Modified: 22 Aug 2016 06:48

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