Influence of interleukin-1 beta on platelet-poor plasma clot formation: A potential impact on early bone healing

Wang, Xin, Luo, Yan, Masci, Paul P., Crawford, Ross, & Xiao, Yin (2016) Influence of interleukin-1 beta on platelet-poor plasma clot formation: A potential impact on early bone healing. PLoS ONE, 11(2), Article Number: -e0149775.

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Abstract

Objectives

Hematoma quality (especially the fibrin matrix) plays an important role in the bone healing process. Here, we investigated the effect of interleukin-1 beta (IL-1β) on fibrin clot formation from platelet-poor plasma (PPP).

Methods

Five-milliliter of rat whole-blood samples were collected from the hepatic portal vein. All blood samples were firstly standardized via a thrombelastograph (TEG), blood cell count, and the measurement of fibrinogen concentration. PPP was prepared by collecting the top two-fifths of the plasma after centrifugation under 400 × g for 10min at 20°C. The effects of IL-1β cytokines on artificial fibrin clot formation from PPP solutions were determined by scanning electronic microscopy (SEM), confocal microscopy (CM), turbidity, and clot lysis assays.

Results

The lag time for protofibril formation was markedly shortened in the IL-1β treatment groups (243.8 ± 76.85 in the 50 pg/mL of IL-1β and 97.5 ± 19.36 in the 500 pg/mL of IL-1β) compared to the control group without IL-1β (543.8 ± 205.8). Maximal turbidity was observed in the control group. IL-1β (500 pg/mL) treatment significantly decreased fiber diameters resulting in smaller pore sizes and increased density of the fibrin clot structure formed from PPP (P < 0.05). The clot lysis assay revealed that 500 pg/mL IL-1β induced a lower susceptibility to dissolution due to the formation of thinner and denser fibers.

Conclusion

IL-1β can significantly influence PPP fibrin clot structure, which may affect the early bone healing process.

Impact and interest:

1 citations in Scopus
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ID Code: 92764
Item Type: Journal Article
Refereed: Yes
Keywords: large bone defect, fracture hematoma, fibrin clot, platelet poor plasma, interleukin-1 beta
DOI: 10.1371/journal.pone.0149775
ISSN: 1932-6203
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: 2016 Wang et al.
Copyright Statement: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Deposited On: 07 Mar 2016 02:05
Last Modified: 08 Mar 2016 05:03

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