Drug discovery approaches utilizing three-dimensional cell culture

Ryan, Sarah-Louise, Baird, Anne-Marie, Vaz, Gisela, Urquhart, Aaron J., Senge, Mathias, Richard, Derek J., O'Byrne, Kenneth J., & Davies, Anthony (2016) Drug discovery approaches utilizing three-dimensional cell culture. ASSAY and Drug Development Technologies, 14(1), pp. 19-28.

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Abstract

Historically, two-dimensional (2D) cell culture has been the preferred method of producing disease models in vitro. Recently, there has been a move away from 2D culture in favor of generating three-dimensional (3D) multicellular structures, which are thought to be more representative of the in vivo environment. This transition has brought with it an influx of technologies capable of producing these structures in various ways. However, it is becoming evident that many of these technologies do not perform well in automated in vitro drug discovery units. We believe that this is a result of their incompatibility with high-throughput screening (HTS). In this study, we review a number of technologies, which are currently available for producing in vitro 3D disease models. We assess their amenability with high-content screening and HTS and highlight our own work in attempting to address many of the practical problems that are hampering the successful deployment of 3D cell systems in mainstream research.

Impact and interest:

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2 citations in Web of Science®

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ID Code: 93034
Item Type: Journal Article
Refereed: Yes
Keywords: 3D culture, Assay Development
DOI: 10.1089/adt.2015.670
ISSN: 1557-8127
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2016 Mary Ann Liebert, Inc.
Deposited On: 18 Feb 2016 02:52
Last Modified: 19 Feb 2016 01:00

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