Higher-resolution structure of the human insulin receptor ectodomain: Multi-modal inclusion of the insert domain

Croll, Tristan I., Smith, Brian J., Margetts, Mai B., Whittaker, Jonathan, Weiss, Michael A., Ward, Colin W., Lawrence, Michael C., , & (2016) Higher-resolution structure of the human insulin receptor ectodomain: Multi-modal inclusion of the insert domain. Structure, 24(3), pp. 469-476.

View at publisher

Abstract

Insulin receptor (IR) signaling is critical to controlling nutrient uptake and metabolism. However, only a low-resolution (3.8 Å) structure currently exists for the IR ectodomain, with some segments ill-defined or unmodeled due to disorder. Here, we revise this structure using new diffraction data to 3.3 Å resolution that allow improved modeling of the N-linked glycans, the first and third fibronectin type III domains, and the insert domain. A novel haptic interactive molecular dynamics strategy was used to aid fitting to low-resolution electron density maps. The resulting model provides a foundation for investigation of structural transitions in IR upon ligand binding.

Impact and interest:

5 citations in Scopus
Search Google Scholar™
5 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 93678
Item Type: Journal Article
Refereed: Yes
DOI: 10.1016/j.str.2015.12.014
ISSN: 0969-2126
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Facilities: HPC – QUT Supercomputer
Deposited On: 14 Mar 2016 03:14
Last Modified: 22 Aug 2016 03:05

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page