Higher-resolution structure of the human insulin receptor ectodomain: Multi-modal inclusion of the insert domain

Croll, Tristan I., Smith, Brian J., Margetts, Mai B., Whittaker, Jonathan, Weiss, Michael A., Ward, Colin W., Lawrence, Michael C., , & (2016) Higher-resolution structure of the human insulin receptor ectodomain: Multi-modal inclusion of the insert domain. Structure, 24(3), pp. 469-476.

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Insulin receptor (IR) signaling is critical to controlling nutrient uptake and metabolism. However, only a low-resolution (3.8 Å) structure currently exists for the IR ectodomain, with some segments ill-defined or unmodeled due to disorder. Here, we revise this structure using new diffraction data to 3.3 Å resolution that allow improved modeling of the N-linked glycans, the first and third fibronectin type III domains, and the insert domain. A novel haptic interactive molecular dynamics strategy was used to aid fitting to low-resolution electron density maps. The resulting model provides a foundation for investigation of structural transitions in IR upon ligand binding.

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8 citations in Scopus
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7 citations in Web of Science®

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ID Code: 93678
Item Type: Journal Article
Refereed: Yes
DOI: 10.1016/j.str.2015.12.014
ISSN: 0969-2126
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Facilities: HPC – QUT Supercomputer
Deposited On: 14 Mar 2016 03:14
Last Modified: 22 Aug 2016 03:05

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