Trehalose accumulation triggers autophagy during plant desiccation

Williams, Brett, Njaci, Isaac, Moghaddam, Lalehvash, Long, Hao, Dickman, Martin B., Zhang, Xiuren, & Mundree, Sagadevan (2015) Trehalose accumulation triggers autophagy during plant desiccation. PLoS Genetics, 11(12), Article Number:-e1005705.

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Abstract

Global climate change, increasingly erratic weather and a burgeoning global population are significant threats to the sustainability of future crop production. There is an urgent need for the development of robust measures that enable crops to withstand the uncertainty of climate change whilst still producing maximum yields. Resurrection plants possess the unique ability to withstand desiccation for prolonged periods, can be restored upon watering and represent great potential for the development of stress tolerant crops. Here, we describe the remarkable stress characteristics of Tripogon loliiformis, an uncharacterised resurrection grass and close relative of the economically important cereals, rice, sorghum, and maize. We show that T. loliiformis survives extreme environmental stress by implementing autophagy to prevent Programmed Cell Death. Notably, we identified a novel role for trehalose in the regulation of autophagy in T.loliiformis. Transcriptome, Gas Chromatography Mass Spectrometry, immunoblotting and confocal microscopy analyses directly linked the accumulation of trehalose with the onset of autophagy in dehydrating and desiccated T. loliiformis shoots. These results were supported in vitro with the observation of autophagosomes in trehalose treated T. loliiformis leaves; autophagosomes were not detected in untreated samples. Presumably, once induced, autophagy promotes desiccation tolerance in T.loliiformis , by removal of cellular toxins to suppress programmed cell death and the recycling of nutrients to delay the onset of senescence. These findings illustrate how resurrection plants manipulate sugar metabolism to promote desiccation tolerance and may provide candidate genes that are potentially useful for the development of stress tolerant crops.

Impact and interest:

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6 citations in Web of Science®

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ID Code: 93941
Item Type: Journal Article
Refereed: Yes
Keywords: Autophagy, Apoptosis, Resurrection Plant, Trehalose, Senescence, Abiotic Stress, Extremophile, Drought, Grass
DOI: 10.1371/journal.pgen.1005705
ISSN: 1553-7404
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Cell Development Proliferation and Death (060103)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700) > Plant Cell and Molecular Biology (060702)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700) > Plant Physiology (060705)
Divisions: Current > Research Centres > Centre for Tropical Crops and Biocommodities
Current > Schools > School of Earth, Environmental & Biological Sciences
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Funding:
  • QUT/Capacity Develop. Profess.Grant-Mundree
  • QUT/VCRF award - Williams
Facilities: Science and Engineering Centre, Central Analytical Research Facility, HPC – QUT Supercomputer
Deposited On: 20 Mar 2016 23:15
Last Modified: 22 Mar 2016 00:47

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