Diverse populations of T cells with NK cell receptors accumulate in the human intestine in health and in colorectal cancer

O'Keeffe, Joan, Doherty, Derek G., Kenna, Tony, Sheahan, Kieran, O'Donoghue, Diarmuid P., Hyland, John M., & O'Farrelly, Cliona (2004) Diverse populations of T cells with NK cell receptors accumulate in the human intestine in health and in colorectal cancer. European Journal of Immunology, 34(8), pp. 2110-2119.

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Abstract

T cells expressing NK cell receptors (NKR) display rapid MHC-unrestricted cytotoxicity and potent cytokine secretion and are thought to play roles in immunity against tumors. We have quantified and characterized NKR+ T cells freshly isolated from epithelial and lamina propria layers of duodenum and colon from 16 individuals with no evidence of gastrointestinal disease and from tumor and uninvolved tissue from 19 patients with colorectal cancer. NKR+ T cell subpopulations were differentially distributed in different intestinal compartments, and CD161+ T cells accounted for over one half of T cells at all locations tested. Most intestinal CD161+ T cells expressed alpha beta TCR and either CD4 or CD8. Significant proportions expressed HLA-DR,CD69 and Fas ligand. Upon stimulation in vitro, CD161+ T cells produced IFN-gamma and TNF-alpha but not IL-4. NKT cells expressing the Valpha24Vbeta11 TCR, which recognizes CD1d,were virtually absent from the intestine, but colonic cells produced IFN-gamma in response to the NKT cell agonist ligand alpha-galactosylceramide. NKR+ T cells were not expanded in colonic tumors compared to adjacent uninvolved tissue. The predominance, heterogeneity and differential distribution of NKR+ T cells at different intestinal locations suggests that they are central to intestinal immunity.

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ID Code: 94069
Item Type: Journal Article
Refereed: Yes
Keywords: Human, Intestine, NKT cells, T lymphocytes, Tumor immunity, alpha galactosylceramide, CD161 antigen, CD1d antigen, CD4 antigen, CD69 antigen, CD8 antigen, cytokine, FAS ligand, gamma interferon, HLA DR antigen, interleukin 4, lymphocyte antigen, lymphocyte receptor, natural killer cell receptor, T lymphocyte receptor alpha chain, T lymphocyte receptor beta chain, tumor necrosis factor alpha, unclassified drug, immunoglobulin receptor, lectin, membrane antigen, adult, aged, article, cell compartmentalization, cell isolation, cellular distribution, clinical article, colon, colorectal cancer, controlled study, cytokine production, cytokine release, duodenum, epithelium cell, female, human cell, human tissue, in vitro study, major histocompatibility complex, male, natural killer cell mediated cytotoxicity, phenotype, priority journal, protein expression, quantitative analysis, T lymphocyte subpopulation, colorectal tumor, immunology, metabolism, T lymphocyte, Antigens, Surface, Colorectal Neoplasms, Cytokines, Humans, Intestines, Lectins, C-Type, Receptors, Immunologic, T-Lymphocytes
DOI: 10.1002/eji.200424958
ISSN: 1521-4141
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 1999-2016 John Wiley & Sons, Inc.
Deposited On: 21 Mar 2016 07:27
Last Modified: 22 Mar 2016 06:07

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