Ultrastructural features of the early secretory pathway in Trichoderma reesei

Nykänen, Marko, Birch, Debra, Peterson, Robyn, Yu, Hong, Kautto, Liisa, Gryshyna, Anna, Te’o, Junior, & Nevalainen, Helena (2016) Ultrastructural features of the early secretory pathway in Trichoderma reesei. Current Genetics, 62(2), pp. 455-465.

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We have systematically analysed the ultra structure of the early secretory pathway in the Trichoderma reesei hyphae in the wild-type QM6a, cellulase overexpressing Rut-C30 strain and a Rut-C30 transformant BV47 overexpressing a recombinant BiP1-VenusYFP fusion protein with an endoplasmic reticulum (ER) retention signal. The hyphae were studied after 24h of growth using transmission electron microscopy, confocal microscopy and quantitative stereological techniques. All three strains exhibited different spatial organisation of the ER at 24h in both a cellulase-inducing medium and a minimal medium containing glycerol as a carbon source (non-cellulase-inducing medium). The wild-type displayed a number of ER subdomains including parallel tubular/cisternal ER, ER whorls, ER-isolation membrane complexes with abundant autophagy vacuoles and dense bodies. Rut-C30 and its transformant BV47 overexpressing the BiP1-VenusYFP fusion protein also contained parallel tubular/cisternal ER, but no ER whorls; also, there were very few autophagy vacuoles and an increasing amount of punctate bodies where particularly the recombinant BiP1-VenusYFPfusion protein was localised. The early presence of distinct strain-specific features such as the dominance of ER whorls in the wild type and tub/cis ER in Rut-C30 suggests that these are inherent traits and not solely a result of cellular response mechanisms by the high secreting mutant to protein overload.

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ID Code: 94081
Item Type: Journal Article
Refereed: Yes
Keywords: Trichoderma reesei, Early secretory compartment, ER subdomains, TEM, Stereology
DOI: 10.1007/s00294-015-0555-1
ISSN: 1432-0983
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2015 Springer-Verlag Berlin Heidelberg
Deposited On: 22 Mar 2016 23:40
Last Modified: 16 May 2016 01:59

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