Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs
Costa, Pedro F., Hutmacher, Dietmar W., Theodoropoulos, Christina, Gomes, Manuela E., Reis, Rui L., & Vaquette, Cedryck (2015) Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs. Advanced Healthcare Materials, 4(6), pp. 864-873.
The ability to test large arrays of cell and biomaterial combinations in 3D environments is still rather limited in the context of tissue engineering and regenerative medicine. This limitation can be generally addressed by employing highly automated and reproducible methodologies. This study reports on the development of a highly versatile and upscalable method based on additive manufacturing for the fabrication of arrays of scaffolds, which are enclosed into individualized perfusion chambers. Devices containing eight scaffolds and their corresponding bioreactor chambers are simultaneously fabricated utilizing a dual extrusion additive manufacturing system. To demonstrate the versatility of the concept, the scaffolds, while enclosed into the device, are subsequently surface-coated with a biomimetic calcium phosphate layer by perfusion with simulated body fluid solution. 96 scaffolds are simultaneously seeded and cultured with human osteoblasts under highly controlled bidirectional perfusion dynamic conditions over 4 weeks. Both coated and noncoated resulting scaffolds show homogeneous cell distribution and high cell viability throughout the 4 weeks culture period and CaP-coated scaffolds result in a significantly increased cell number. The methodology developed in this work exemplifies the applicability of additive manufacturing as a tool for further automation of studies in the field of tissue engineering and regenerative medicine.
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|Item Type:||Journal Article|
|Keywords:||Tissue engineering, 3D tissue engineered constructs, additively manufactured device, dynamic culture of large arrays, cell and biomaterial combinations, scaffolds, bioreactor chambers, dual extrusion additive manufacturing system, regenerative medicine|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Structural Biology (incl. Macromolecular Modelling) (060112)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Synthetic Biology (060113)
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Owner:||© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim|
|Deposited On:||11 Apr 2016 23:15|
|Last Modified:||12 Apr 2016 21:43|
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