Three-dimensional organization of fenestrae labyrinths in liver sinusoidal endothelial cells

Braet, Filip, Riches, James, Geerts, Willie, Jahn, Kristina A., Wisse, Eddie, & Frederik, Peter (2009) Three-dimensional organization of fenestrae labyrinths in liver sinusoidal endothelial cells. Liver International, 29(4), pp. 603-613.

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  • Background/Aims

Liver sinusoidal endothelial cell (LSEC) fenestrae are membrane-bound pores that are grouped in sieve plates and act as a bidirectional guardian in regulating transendothelial liver transport. The high permeability of the endothelial lining is explained by the presence of fenestrae and by various membrane-bound transport vesicles. The question as to whether fenestrae relate to other transport compartments remains unclear and has been debated since their discovery almost 40 years ago.

  • Methods

In this study, novel insights concerning the three-dimensional (3D) organization of the fenestrated cytoplasm were built on transmission electron tomographical observations on isolated and cultured whole-mount LSECs. Classical transmission electron microscopy and atomic force microscopy imaging was performed to accumulate cross-correlative structural evidence.

  • Results and Conclusions

The data presented here indicate that different arrangements of fenestrae have to be considered: i.e. open fenestrae that lack any structural obstruction mainly located in the thin peripheral cytoplasm and complexes of multifolded fenestrae organized as labyrinth-like structures that are found in the proximity of the perinuclear area. Fenestrae in labyrinths constitute about one-third of the total LSEC porosity. The 3D reconstructions also revealed that coated pits and small membrane-bound vesicles are exclusively interspersed in the non-fenestrated cytoplasmic arms.

Impact and interest:

16 citations in Scopus
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14 citations in Web of Science®

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ID Code: 94943
Item Type: Journal Article
Refereed: Yes
Keywords: atomic force microscopy;electron tomography;fenestrae labyrinth;fenestrations;hepatic endothelium;liver sieve;liver sinusoidal endothelium;pores;porosity;TEM sections;transendothelial transport
DOI: 10.1111/j.1478-3231.2008.01836.x
ISSN: 1478-3231
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 18 Apr 2016 22:51
Last Modified: 20 Apr 2016 04:44

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