Involvement of Ca2+-dependent hyperpolarization in sleep duration in mammals
Tatsuki, Fumiya, Sunagawa, Genshiro A., Shi, Shoi, Susaki, Etsuo A., Yukinaga, Hiroko, Perrin, Dimitri, Sumiyama, Kenta, Ukai-Tadenuma, Maki, Fujishima, Hiroshi, Ohno, Rei-ichiro, Tone, Daisuke, Ode, Koji L., Matsumoto, Katsuhiko, & Ueda, Hiroki R. (2016) Involvement of Ca2+-dependent hyperpolarization in sleep duration in mammals. Neuron, 90(1), pp. 70-85.
Administrators only until April 2017 | Request a copy from author
Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0.
The detailed molecular mechanisms underlying the regulation of sleep duration in mammals are still elusive. To address this challenge, we constructed a simple computational model, which recapitulates the electrophysiological characteristics of the slow-wave sleep and awake states. Comprehensive bifurcation analysis predicted that a Ca2+-dependent hyperpolarization pathway may play a role in slow-wave sleep and hence in the regulation of sleep duration. To experimentally validate the prediction, we generate and analyze 21 KO mice. Here we found that impaired Ca2+-dependent K+ channels (Kcnn2 and Kcnn3), voltage-gated Ca2+ channels (Cacna1g and Cacna1h), or Ca2+/calmodulin-dependent kinases (Camk2a and Camk2b) decrease sleep duration, while impaired plasma membrane Ca2+ ATPase (Atp2b3) increases sleep duration. Pharmacological intervention and whole-brain imaging validated that impaired NMDA receptors reduce sleep duration and directly increase the excitability of cells. Based on these results, we propose a hypothesis that a Ca2+-dependent hyperpolarization pathway underlies the regulation of sleep duration in mammals.
Impact and interest:
Citation counts are sourced monthly from and citation databases.
These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.
Citations counts from theindexing service can be viewed at the linked Google Scholar™ search.
|Item Type:||Journal Article|
|Divisions:||Current > Schools > School of Electrical Engineering & Computer Science
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2016 Elsevier|
|Copyright Statement:||Licensed under the Creative Commons Attribution; Non-Commercial; No-Derivatives 4.0 International. DOI: 10.1016/j.neuron.2016.02.032|
|Deposited On:||28 Apr 2016 22:36|
|Last Modified:||20 May 2016 10:39|
Repository Staff Only: item control page