Synthesis of 19F nucleic acid–polymer conjugates as real-time MRI probes of biorecognition

Sicilia, Giovanna, Davis, Adrienne L., Spain, Sebastian G., Magnusson, Johannes P., Boase, Nathan R.B., Thurecht, Kristofer J., & Alexander, Cameron (2016) Synthesis of 19F nucleic acid–polymer conjugates as real-time MRI probes of biorecognition. Polymer Chemistry, 7(12), pp. 2180-2191.

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Abstract

Polymer-DNA conjugates in which one nucleic acid strand contains fluorine-substituted nucleobases have been prepared and characterised. The efficacy of these novel F-19 nucleic acid-polymer conjugates as sensitive and selective in vitro reporters of DNA binding events is demonstrated through a number of rapid-acquisition MR sequences. The conjugates respond readily and in a sequence specific manner to external target oligonucleotide sequences by changes in hybridisation. In turn, these structural changes in polymer-nucleotide conjugates translate into responses which are detectable in fluorine relaxation and diffusion switches, and which can be monitored by in vitro Spin Echo and DOSY NMR spectroscopy. Although complementary to conventional FRET methods, the excellent diagnostic properties of fluorine nuclei make this approach a versatile and sensitive probe of molecular structure and conformation in polymeric assemblies.

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ID Code: 95445
Item Type: Journal Article
Refereed: Yes
DOI: 10.1039/c5py01883h
ISSN: 1759-9962
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 10 May 2016 01:56
Last Modified: 10 May 2016 22:46

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