Unusual RNA plant virus integration in the soybean genome leads to the production of small RNAs

Cordenonsi da Fonseca, Guilherme, Valter de Oliveira, Luis Felipe, Loss de Morais, Guilherme, Abdelnor, Ricardo Vilela, Nepomuceno, Alexandre Lima, Waterhouse, Peter M., Farinelli, Laurent, & Margis, Rogerio (2016) Unusual RNA plant virus integration in the soybean genome leads to the production of small RNAs. Plant Science, 246, pp. 62-69.

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Horizontal gene transfer (HGT) is known to be a major force in genome evolution. The acquisition of genes from viruses by eukaryotic genomes is a well-studied example of HGT, including rare cases of non-retroviral RNA virus integration. The present study describes the integration of cucumber mosaic virus RNA-1 into soybean genome. After an initial metatranscriptomic analysis of small RNAs derived from soybean, the de novo assembly resulted a 3029-nt contig homologous to RNA-1. The integration of this sequence in the soybean genome was confirmed by DNA deep sequencing. The locus where the integration occurred harbors the full RNA-1 sequence followed by the partial sequence of an endogenous mRNA and another sequence of RNA-1 as an inverted repeat and allowing the formation of a hairpin structure. This region recombined into a retrotransposon located inside an exon of a soybean gene. The nucleotide similarity of the integrated sequence compared to other Cucumber mosaic virus sequences indicates that the integration event occurred recently. We described a rare event of non-retroviral RNA virus integration in soybean that leads to the production of a double-stranded RNA in a similar fashion to virus resistance RNAi plants.

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2 citations in Web of Science®

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ID Code: 95500
Item Type: Journal Article
Refereed: Yes
Keywords: Horizontal gene transfer, RNA plant virus, Cucumber mosaic virus, Soybean, Transposon
DOI: 10.1016/j.plantsci.2016.01.011
ISSN: 0168-9452
Divisions: Current > Research Centres > Centre for Tropical Crops and Biocommodities
Current > Schools > School of Earth, Environmental & Biological Sciences
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2016 Elsevier Ireland Ltd.
Deposited On: 11 May 2016 23:14
Last Modified: 13 May 2016 00:57

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