Comparison of methods for dextran analysis: Effect of sugar and dextran concentrations

Ninchan, B., Saothong, P., Slrisatesuwcm, C., Jangchud, A., & Doherty, W.O.S. (2016) Comparison of methods for dextran analysis: Effect of sugar and dextran concentrations. International Sugar Journal, 118(1408), pp. 260-267.

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The presence of dextran in the process streams of a sugar factory is an immediate indicator of sugar loss. In the present study, four methods used for dextran determination in sugar factory process streams were assessed -the Haze method, the Modified Haze I method, the Modified Haze II method, and enzyme-linked immunosorbent or ELISA method. All four methods were both accurate and reproducible in the range 10-100 ug/ml dextran in pure dextran solutions. Only the Modified Haze I and II methods gave acceptable results in the range 100-1000 ug/ml dextran in dextran solutions and in dextran solutions containing 15 % sucrose. The two methods were also able to give accepted results with factory samples diluted to 15 Brix. However, the factory samples should be diluted to dextran concentrations between 100 and 5000 ug/ml dextran prior to analysis. As this study established that sucrose interferes with haze formation, the Modified Haze II method is the preferred method for routine dextran analysis in a sugar factory. However, further studies of the Modified Haze II method are suggested.

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ID Code: 95997
Item Type: Journal Article
Refereed: Yes
Keywords: haze method, modified haze method, dextran determination, ELISA
ISSN: 0020-8841
Divisions: Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2016 Informa Agra
Deposited On: 08 Jun 2016 22:31
Last Modified: 27 Jun 2017 22:01

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