The repeatability of an oral glucose test in ponies

de Laat, Melody A. & Sillence, Martin (2016) The repeatability of an oral glucose test in ponies. Equine Veterinary Journal. (In Press)

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Abstract

Reasons for performing study

  • Insulin dysregulation can be difficult to diagnose from basal insulin and glucose concentrations, so a field-based oral glucose test (OGT) is preferred. However, the repeatability of this test has not been reported.

Objectives

  • To determine the repeatability of an in-feed OGT in ponies and examine some factors affecting the palatability of the test meal.

Study design

  • A repeated measures, longitudinal study.

Methods

  • An in-feed OGT was performed at 08.00 h on 3 consecutive occasions under controlled conditions in 8 mixed breed ponies. d-glucose (0.75 g/kg bwt) was dissolved in water and combined with wheat bran and lucerne chaff. Blood samples were taken before and 90, 120, 180 min and 24 h after d-glucose. The repeatability of the test was analysed with repeated measures ANOVA. Insulin and glucose responses to d-glucose were also compared to an equivalent dose of dietary carbohydrate provided with a commercial grain mixture.

Results

  • The overall insulin responses to the OGTs did not differ between tests. Individual insulin responses were more variable (P<0.05) than glucose responses. There was no difference in insulin concentration in post d-glucose samples over time. Insulin and glucose responses to grain and d-Glucose were not different.

Conclusions

  • An OGT is reasonably repeatable in ponies. The currently recommended post-glucose sampling time point of 2 h is acceptable, with sampling at 90 min also likely to produce a consistent result. The use of an alternative carbohydrate source to d-glucose, such as a commercial grain-based product, may be a viable and more palatable option for the test.

Impact and interest:

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ID Code: 96273
Item Type: Journal Article
Refereed: Yes
Keywords: horse, insulin, hyperinsulinaemia, equine metabolic syndrome, laminitis, grain
DOI: 10.1111/evj.12579
ISSN: 0425-1644
Subjects: Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000) > VETERINARY SCIENCES (070700) > Veterinary Medicine (070706)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2016 EVJ Ltd
Deposited On: 20 Jun 2016 23:06
Last Modified: 23 Jun 2016 05:47

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