The repeatability of an oral glucose test in ponies

de Laat, Melody A. & Sillence, Martin (2016) The repeatability of an oral glucose test in ponies. Equine Veterinary Journal. (In Press)

[img] Accepted Version (PDF 703kB)
Administrators only | Request a copy from author

View at publisher


Reasons for performing study

  • Insulin dysregulation can be difficult to diagnose from basal insulin and glucose concentrations, so a field-based oral glucose test (OGT) is preferred. However, the repeatability of this test has not been reported.


  • To determine the repeatability of an in-feed OGT in ponies and examine some factors affecting the palatability of the test meal.

Study design

  • A repeated measures, longitudinal study.


  • An in-feed OGT was performed at 08.00 h on 3 consecutive occasions under controlled conditions in 8 mixed breed ponies. d-glucose (0.75 g/kg bwt) was dissolved in water and combined with wheat bran and lucerne chaff. Blood samples were taken before and 90, 120, 180 min and 24 h after d-glucose. The repeatability of the test was analysed with repeated measures ANOVA. Insulin and glucose responses to d-glucose were also compared to an equivalent dose of dietary carbohydrate provided with a commercial grain mixture.


  • The overall insulin responses to the OGTs did not differ between tests. Individual insulin responses were more variable (P<0.05) than glucose responses. There was no difference in insulin concentration in post d-glucose samples over time. Insulin and glucose responses to grain and d-Glucose were not different.


  • An OGT is reasonably repeatable in ponies. The currently recommended post-glucose sampling time point of 2 h is acceptable, with sampling at 90 min also likely to produce a consistent result. The use of an alternative carbohydrate source to d-glucose, such as a commercial grain-based product, may be a viable and more palatable option for the test.

Impact and interest:

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 96273
Item Type: Journal Article
Refereed: Yes
Keywords: horse, insulin, hyperinsulinaemia, equine metabolic syndrome, laminitis, grain
DOI: 10.1111/evj.12579
ISSN: 0425-1644
Subjects: Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000) > VETERINARY SCIENCES (070700) > Veterinary Medicine (070706)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2016 EVJ Ltd
Deposited On: 20 Jun 2016 23:06
Last Modified: 23 Jun 2016 05:47

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page