hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress
Paquet, Nicolas, Adams, Mark N., Ashton, Nicholas W., Touma, Christine, Gamsjaeger, Roland, Cubeddu, Liza, Leong, Vincent, Beard, Sam, Bolderson, Emma, Botting, Catherine H., O'Byrne, Kenneth J., & Richard, Derek J. (2016) hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress. Scientific Reports, 6, Article number: 27446.
The maintenance of genome stability is an essential cellular process to prevent the development of diseases including cancer. hSSB1 (NABP2/ OBFC2A) is a critical component of the DNA damage response where it participates in the repair of double-strand DNA breaks and in base excision repair of oxidized guanine residues (8-oxoguanine) by aiding the localization of the human 8-oxoguanine glycosylase (hOGG1) to damaged DNA. Here we demonstrate that following oxidative stress, hSSB1 is stabilized as an oligomer which is required for hSSB1 to function in the removal of 8-oxoguanine. Monomeric hSSB1 shows a decreased affinity for oxidized DNA resulting in a cellular 8-oxoguanine-repair defect and in the absence of ATM signaling initiation. While hSSB1 oligomerization is important for the removal of 8-oxoguanine from the genome, it is not required for the repair of double-strand DNA-breaks by homologous recombination. These findings demonstrate a novel hSSB1 regulatory mechanism for the repair of damaged DNA.
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|Item Type:||Journal Article|
|Keywords:||hSSB1, oxidative stress, 8-oxoguanine, Base excision repair, hOGG1|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400) > Genome Structure and Regulation (060407)|
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Statement:||This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/|
|Deposited On:||22 Jun 2016 22:29|
|Last Modified:||27 Jun 2016 23:59|
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