Sequential collision- and ozone-induced dissociation enables assignment of relative acyl chain position in triacylglycerols

Marshall, David L., Pham, Huong T., Bhujel, Mahendra, Chin, Jacqueline S.R., Yew, Joanne Y., Mori, Kenji, Mitchell, Todd W., & Blanksby, Stephen J. (2016) Sequential collision- and ozone-induced dissociation enables assignment of relative acyl chain position in triacylglycerols. Analytical Chemistry, 88(5), pp. 2685-2692.

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Abstract

Unambiguous identification of isomeric lipids by mass spectrometry represents a significant analytical challenge in contemporary lipidomics. Herein, the combination of collision-induced dissociation (CID) with ozone-induced dissociation (OzID) on an ion-trap mass spectrometer is applied to the identification of triacylglycerol (TG) isomers that vary only by the substitution pattern of fatty acyl (FA) chains esterified to the glycerol backbone. Isolated product ions attributed to loss of a single FA arising from CID of [TG + Na]+ ions react rapidly with ozone within the ion trap. The resulting CID/OzID spectra exhibit abundant ions that unequivocally reveal the relative position of FAs along the backbone. Isomeric TGs containing two or three different FA substituents are readily differentiated by diagnostic ions present in their CID/OzID spectra. Compatibility of this method with chromatographic separations enables the characterization of unusual TGs containing multiple short-chain FAs present in Drosophila.

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4 citations in Scopus
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2 citations in Web of Science®

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ID Code: 96422
Item Type: Journal Article
Refereed: Yes
Keywords: Chains, Dissociation, Glycerol, Isomers, Liquid chromatography, Mass spectrometers, Mass spectrometry, Ozone, Analytical challenge, Chromatographic separations, Collision induced dissociation, Induced dissociation, Ion trap mass spectrometer, Relative positions, Sequential collisions, Substitution patterns, Ions
DOI: 10.1021/acs.analchem.5b04001
ISSN: 0003-2700
Subjects: Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > ANALYTICAL CHEMISTRY (030100)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Funding:
Facilities: Central Analytical Research Facility
Copyright Owner: Copyright 2016 American Chemical Society
Deposited On: 30 Jun 2016 05:19
Last Modified: 08 Feb 2017 20:35

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