Influence of meibomian gland expression methods on human lipid analysis results
Kunnen, Carolina M.E., Brown, Simon H.J., de la Jara, Percy Lazon, Holden, Brien A., Blanksby, Stephen J., Mitchell, Todd W., & Papas, Eric B. (2016) Influence of meibomian gland expression methods on human lipid analysis results. The Ocular Surface, 14(1), pp. 49-55.
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Purpose To compare the lipid composition of human meibum across three different meibum expression techniques. Methods Meibum was collected from five healthy non-contact lens wearers (aged 20-35 years) after cleaning the eyelid margin using three meibum expression methods: cotton buds (CB), meibomian gland evaluator (MGE) and meibomian gland forceps (MGF). Meibum was also collected using cotton buds without cleaning the eyelid margin (CBn). Lipids were analyzed by chip-based, nano-electrospray mass spectrometry (ESI-MS). Comparisons were made using linear mixed models. Results Tandem MS enabled identification and quantification of over 200 lipid species across ten lipid classes. There were significant differences between collection techniques in the relative quantities of polar lipids obtained (P<.05). The MGE method returned smaller polar lipid quantities than the CB approaches. No significant differences were found between techniques for nonpolar lipids. No significant differences were found between cleaned and non-cleaned eyelids for polar or nonpolar lipids. Conclusion Meibum expression technique influences the relative amount of phospholipids in the resulting sample. The highest amounts of phospholipids were detected with the CB approaches and the lowest with the MGE technique. Cleaning the eyelid margin prior to expression was not found to affect the lipid composition of the sample. This may be a consequence of the more forceful expression resulting in cell membrane contamination or higher risk of tear lipid contamination as a result of reflex tearing.© 2016 Elsevier Inc.
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|Item Type:||Journal Article|
|Keywords:||lipids, mass spectrometry, meibomian gland, meibum, meibum expression, phospholipids, phospholipid, adult, Article, cell membrane, clinical article, controlled study, electrospray mass spectrometry, female, human, intermethod comparison, lipid analysis, lipid composition, male, meibomian gland, nano electrospray mass spectrometry, non contact lens, ophthalmological therapeutic device, standard, visual acuity chart|
|Subjects:||Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > ANALYTICAL CHEMISTRY (030100)|
|Divisions:||Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2016 Elsevier|
|Copyright Statement:||Licensed under the Creative Commons Attribution; Non-Commercial; No-Derivatives 4.0 International. DOI: 10.1016/j.jtos.2015.10.001|
|Deposited On:||30 Jun 2016 01:32|
|Last Modified:||20 Jul 2016 04:19|
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