Intersubject and interday variability in human tear and meibum lipidomes: A pilot study

Brown, Simon H.J., Kunnen, Carolina M.E., Papas, Eric B., de la Jara, Percy Lazon, Willcox, Mark D.P., Blanksby, Stephen J., & Mitchell, Todd W. (2016) Intersubject and interday variability in human tear and meibum lipidomes: A pilot study. The Ocular Surface, 14(1), pp. 43-48.

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Purpose Our aim was to quantitate day-to-day changes in the tear and meibum lipid profile of individual subjects in a pilot study of healthy humans. Methods Matched tear and meibum samples were obtained from four subjects on three consecutive days. Quantitative lipid profiles of human basal tears and meibum were compared using multivariate analysis by principal components. Results Substantial differences in the lipid profile between subjects were observed, while lipid profiles were steady across the three consecutive days of sampling. Multivariate principal component analysis demonstrated that lysophosphatidylcholine was the largest variant lipid class between subjects in tears, while wax esters comprised the most variation between subjects in meibum secretions. Conclusion Interday variability is shown to be much smaller than interpatient variability, suggesting that tears and meibum subjects both have unique profiles in humans.

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5 citations in Scopus
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5 since deposited on 30 Jun 2016
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ID Code: 96425
Item Type: Journal Article
Refereed: Yes
Keywords: lipidomics, lipids, meibum, multivariate analysis, tear, cholesterol ester, ester derivative, lipidome, lysophosphatidylcholine, meibum, phospholipid, unclassified drug, wax ester, adult, Article, controlled study, human, lacrimal fluid, lipid analysis, lipid composition, lipidomics, lipogenesis, pilot study, principal component analysis, signal transduction
DOI: 10.1016/j.jtos.2015.08.005
ISSN: 1542-0124
Subjects: Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > ANALYTICAL CHEMISTRY (030100)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2016 Elsevier
Copyright Statement: Licensed under the Creative Commons Attribution; Non-Commercial; No-Derivatives 4.0 International. DOI: 10.1016/j.jtos.2015.08.005
Deposited On: 30 Jun 2016 04:23
Last Modified: 08 Feb 2017 20:38

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