Combining liquid chromatography with ozone-induced dissociation for the separation and identification of phosphatidylcholine double bond isomers
Kozlowski, Rachel L., Campbell, J.Larry, Mitchell, Todd W., & Blanksby, Stephen J. (2015) Combining liquid chromatography with ozone-induced dissociation for the separation and identification of phosphatidylcholine double bond isomers. Analytical and Bioanalytical Chemistry, 407(17), pp. 5053-5064.
Revealing the inherent molecular diversity of lipid biology requires advanced analytical technologies. Distinguishing phospholipids that differ in the position(s) of carbon-carbon double bonds within their acyl chains presents a particular challenge because of their similar chromatographic and mass spectral behaviours. Here or the first time we combine reversed-phase liquid chromatography for separation of isomeric phospholipids with on-line mass spectral analysis by ozone-induced dissociation (OzID) for unambiguous double bond position assignment. The customised tandem linear ion-trap mass spectrometer used in our study is capable of acquiring OzID scans on a chromatographic timescale. Resolving the contributions of isomeric lipids that are indistinguishable based on conventional mass spectral analysis is achieved using the combination of liquid chromatography and OzID. Application of this method to the analysis of simple (egg yolk) and more complex (sheep brain) extracts reveals significant populations of the phosphatidylcholine PC 16:0_18:1(n-7) alongside the expected PC 16:0_18:1(n-9) isomer.
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|Item Type:||Journal Article|
|Keywords:||Collision-induced dissociation, Double bond isomers, Liquid chromatography, Mass spectrometry, Ozone-induced dissociation, Phospholipids, Carbon, Chromatography, Dissociation, Isomers, Liquids, Mass spectrometers, Mass spectrometry, Ozone, Phospholipids, Plants (botany), Spectrum analysis, Carbon-carbon double bonds, Collision induced dissociation, Double bond positions, Double bonds, Induced dissociation, Mass-spectral analysis, Reversed phase liquid-chromatography, Separation and identification, Liquid chromatography|
|Subjects:||Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > ANALYTICAL CHEMISTRY (030100)
Australian and New Zealand Standard Research Classification
|Divisions:||Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2015 Springer|
|Deposited On:||30 Jun 2016 04:17|
|Last Modified:||13 Jul 2016 10:17|
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