Separation and identification of phosphatidylcholine regioisomers by combining liquid chromatography with a fusion of collision- and ozone-induced dissociation
Kozlowski, Rachel L., Mitchell, Todd W., & Blanksby, Stephen J. (2015) Separation and identification of phosphatidylcholine regioisomers by combining liquid chromatography with a fusion of collision- and ozone-induced dissociation. European Journal of Mass Spectrometry, 21(3), pp. 191-200.
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The differentiation of closely related lipid isomers is increasingly important to our evolving understanding of lipid biochemistry but it is equally challenging to contemporary chromatographic and mass spectral analyses. Recently, we described a novel ion-activation approach based on combining collision- with ozone-induced dissociation (CID/OzID) for the identification of the relative acyl chain substitution positions in glycerophospholipids. Here we demonstrate, for the first time, that CID/OzID can be effectively combined with reversed-phase chromatography to enable the separation and unambiguous identification of regioisomeric pairs of phosphatidylcholines that differ only in the arrangement of acyl chains on the glycerol backbone.
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|Item Type:||Journal Article|
|Additional Information:||Unmapped bibliographic data:
LA - English [Field not mapped to EPrints]
J2 - Eur. J. Mass Spectrom. [Field not mapped to EPrints]
C2 - 26307699 [Field not mapped to EPrints]
AD - School of Chemistry, University of Wollongong, Wollongong, NSW, Australia [Field not mapped to EPrints]
AD - Illawarra Health and Medical Research Institute (IHMRI), University of Wollongong, Wollongong, NSW, Australia [Field not mapped to EPrints]
AD - School of Medicine, University of Wollongong, Wollongong, NSW, Australia [Field not mapped to EPrints]
AD - Central Analytical Research Facility, Queensland University of Technology, Brisbane, QLD, Australia [Field not mapped to EPrints]
DB - Scopus [Field not mapped to EPrints]
M3 - Article [Field not mapped to EPrints]
|Keywords:||Isomers, Lipids, Liquid chromatography, Mass spectrometry, Ozone-induced dissociation, Phosphatidylcholines, ozone, phosphatidylcholine, chemistry, high performance liquid chromatography, isolation and purification, isomerism, mass spectrometry, procedures, Chromatography, High Pressure Liquid, Isomerism, Mass Spectrometry, Ozone, Phosphatidylcholines|
|Subjects:||Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > ANALYTICAL CHEMISTRY (030100)|
|Divisions:||Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2015 IM Publications LLP|
|Deposited On:||30 Jun 2016 03:00|
|Last Modified:||21 Jul 2016 16:52|
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