A hydrogel model incorporating 3D-plotted hydroxyapatite for osteochondral tissue engineering

Bartnikowski, Michal, Akkineni, Ashwini Rahul, Gelinsky, Michael, Woodruff, Maria A., & Klein, Travis J. (2016) A hydrogel model incorporating 3D-plotted hydroxyapatite for osteochondral tissue engineering. Materials, 9(4), Article No. 285.

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Abstract

The concept of biphasic or multi-layered compound scaffolds has been explored within numerous studies in the context of cartilage and osteochondral regeneration. To date, no system has been identified that stands out in terms of superior chondrogenesis, osteogenesis or the formation of a zone of calcified cartilage (ZCC). Herein we present a 3D plotted scaffold, comprising an alginate and hydroxyapatite paste, cast within a photocrosslinkable hydrogel made of gelatin methacrylamide (GelMA), or GelMA with hyaluronic acid methacrylate (HAMA). We hypothesized that this combination of 3D plotting and hydrogel crosslinking would form a high fidelity, cell supporting structure that would allow localization of hydroxyapatite to the deepest regions of the structure whilst taking advantage of hydrogel photocrosslinking. We assessed this preliminary design in terms of chondrogenesis in culture with human articular chondrocytes, and verified whether the inclusion of hydroxyapatite in the form presented had any influence on the formation of the ZCC. Whilst the inclusion of HAMA resulted in a better chondrogenic outcome, the effect of HAP was limited. We overall demonstrated that formation of such compound structures is possible, providing a foundation for future work. The development of cohesive biphasic systems is highly relevant for current and future cartilage tissue engineering.

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ID Code: 96474
Item Type: Journal Article
Refereed: Yes
Keywords: cartilage tissue engineering; hydrogel; 3D plotting; hydroxyapatite; gelatin; alginate; chondrocyte; chondrogenesis
DOI: 10.3390/ma9040285
ISSN: 1996-1944
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Funding:
Copyright Owner: Copyright 2016 by the authors; licensee MDPI, Basel, Switzerland
Copyright Statement: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution
(CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
Deposited On: 30 Jun 2016 23:27
Last Modified: 13 Jul 2016 01:08

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