Validation of a new mixing chamber system for breath-by-breath indirect calorimetry

Kim, D. & Robergs, R.A. (2012) Validation of a new mixing chamber system for breath-by-breath indirect calorimetry. Applied Physiology, Nutrition and Metabolism, 37(1), pp. 157-166.

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Limited validation research exists for applications of breath-by-breath systems of expired gas analysis indirect calorimetry (EGAIC) during exercise. We developed improved hardware and software for breath-by-breath indirect calorimetry (NEW) and validated this system as well as a commercial system (COM) against 2 methods: (i) mechanical ventilation with known calibration gas, and (ii) human subjects testing for 5 min each at rest and cycle ergometer exercise at 100 and 175 W. Mechanical calibration consisted of medical grade and certified calibration gas ((4.95% CO2, 12.01% O2, balance N2), room air (20.95% O2, 0.03% CO2, balance N2), and 100% nitrogen), and an air flow turbine calibrated with a 3-L calibration syringe. Ventilation was mimicked manually using complete 3-L calibration syringe manouvres at a rate of 10·min–1 from a Douglas bag reservoir of calibration gas. The testing of human subjects was completed in a counterbalanced sequence based on 5 repeated tests of all conditions for a single subject. Rest periods of 5 and 10 min followed the 100 and 175 W conditions, respectively. COM and NEW had similar accuracy when tested with known ventilation and gas fractions. However, during human subjects testing COM significantly under-measured carbon dioxide gas fractions, over-measured oxygen gas fractions and minute ventilation, and resulted in errors to each of oxygen uptake, carbon dioxide output, and respiratory exchange ratio. These discrepant findings reveal that controlled ventilation and gas fractions are insufficient to validate breath-by-breath, and perhaps even time-averaged, systems of EGAIC. The errors of the COM system reveal the need for concern over the validity of commercial systems of EGAIC.

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2 citations in Web of Science®
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ID Code: 96786
Item Type: Journal Article
Refereed: Yes
Keywords: article; artificial ventilation; breath analysis; calibration; calorimetry; equipment design; exercise; exercise test; exhalation; human; instrumentation; lung; male; middle aged; physiology; predictive value; reproducibility; standard; time; validation s, Breath Tests; Calibration; Calorimetry, Indirect; Equipment Design; Exercise; Exercise Test; Exhalation; Humans; Lung; Male; Middle Aged; Predictive Value of Tests; Reproducibility of Results; Respiration, Artificial; Time Factors
DOI: 10.1139/H11-147
ISSN: 1715-5312
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Schools > School of Exercise & Nutrition Sciences
Copyright Owner: 2012 N R C Research Press
Deposited On: 26 Jul 2016 04:45
Last Modified: 08 Aug 2016 23:34

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