Identifying plasma glycerol concentration associated with urinary glycerol excretion in trained humans

Nelson, J.L., Harmon, M.E., & Robergs, Robert A. (2011) Identifying plasma glycerol concentration associated with urinary glycerol excretion in trained humans. Journal of Analytical Toxicology, 35(9), pp. 617-623.

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Glycerol has been used as a means to legitimately hyperhydrate the body in an attempt to offset the deleterious effects of dehydration. It has the potential to mask blood doping practices and as a result has been added to theWADA prohibited substance list. The purpose of this study was to identify the plasma glycerol concentration coinciding with urinary glycerol excretion. Twelve healthy, trained male subjects completed five separate trials under resting conditions. For each trial, subjects consumed a different glycerol dose (0.025, 0.05, 0.10, 0.15, or 0.20 g glycerol/kg LBM) of a 5% glycerol solution in order to determine at what plasma glycerol concentration an increase in urine glycerol concentration becomes apparent. Based on regression analysis, plasma glycerol concentrations > 0.327 ± 0.190 mmol/L and a glycerol dose > 0.032 ± 0.010 g glycerol/kg LBM would be associated with urinary glycerol excretion. There were significant linear relationships between peak plasma glycerol concentration and time to reach peak plasma glycerol concentration to the ingested glycerol doses. Our findings illustrate the importance of considering the effect of urinary glycerol excretion on legitimate hyperhydration regimens as well as suggesting that it is possible to detect surreptitious use of glycerol as a masking agent through urinary analysis.

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ID Code: 96788
Item Type: Journal Article
Refereed: Yes
Keywords: glycerol, adult; article; dehydration; doping; drug urine level; glycerol blood level; human; human experiment; hydration; male; maximum plasma concentration; normal human; time to maximum plasma concentration; urinalysis; urinary excretion; urine, Dehydration; Doping in Sports; Dose-Response Relationship, Drug; Glycerol; Humans; Linear Models; Male; Metabolic Clearance Rate; Physical Fitness; Substance Abuse Detection
DOI: 10.1093/anatox/35.9.617
ISSN: 0146-4760
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Schools > School of Exercise & Nutrition Sciences
Copyright Owner: 2011 Oxford University Press
Deposited On: 26 Jul 2016 05:13
Last Modified: 27 Jul 2016 01:50

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