Effects of whole-body heat acclimation on cell injury and cytokine responses in peripheral blood mononuclear cells

Amorim, F., Yamada, P., Robergs, R.A., Schneider, S., & Moseley, P. (2011) Effects of whole-body heat acclimation on cell injury and cytokine responses in peripheral blood mononuclear cells. European Journal of Applied Physiology, 111(8), pp. 1609-1618.

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Abstract

To test the hypothesis that whole-body heat acclimation (HA) would increase peripheral blood mononuclear cells' (PBMC) tolerance to heat shock (HS) and/or alter the release of cytokines (IL-1β, IL-6, IL-10 and TNFa) to bacterial lipopolysaccharide (LPS), we heat acclimated nine subjects by exercising them for 100 min in a hot environment for 10 days. The subjects' PBMC were separated and cultured on days 1 and 10 of HA pre- and post-exercise. Pre-exercise PBMC were allocated to three treatments: control (PRE, 37°C), HS (42.5°C for 2 h), or LPS (1 ng mL -1 for 24 h). Post-exercise samples were incubated at 37°C. PBMC lactate dehydrogenase release increased (p<0.05) after HS but it was not different (p>0.05) between days 1 and 10 (0.100 ± 0.012 and 0.102 ± 0.16 abs., respectively). LPS treatment induced an increased (p<0.05) release of cytokines but HA did not alter this response (p[0.05). Pre-exercise intracellular heat shock protein 72 (Hsp72) was higher (p<0.05) on day 10 compared to day 1 of HA (13 ± 5 and 8 ± 5 ng mL -1, respectively). HS treatment caused a greater increase (p\0.05) in Hsp72 than the exercise sessions on HA days 1 and 10. In addition, after HA, the Hsp72 response to HS was reduced (day 1, 129 ± 46; day 10, 80 ± 32 ng mL -1, p<0.05). In conclusion, HA increases PBMC Hsp72 but it does not reduce cellular damage to HS or alter cytokine response to LPS. We speculate that the stress applied during HA is not sufficient to modify the PBMC response.

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ID Code: 96791
Item Type: Journal Article
Refereed: Yes
Keywords: cytokine, acclimatization; adult; article; cell culture; clinical trial; exercise; exercise test; female; heat; heat shock response; human; male; metabolism; microclimate; mononuclear cell; pathology; physiology; thermoregulation, Acclimatization; Adult; Body Temperature Regulation; Cells, Cultured; Cytokines; Environment, Controlled; Exercise; Exercise Test; Female; Heat-Shock Response; Hot Temperature; Humans; Leukocytes, Mononuclear; Male; Young Adult
DOI: 10.1007/s00421-010-1780-4
ISSN: 1439-6319
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Schools > School of Exercise & Nutrition Sciences
Copyright Owner: 2011 European Journal of Applied Physiology
Deposited On: 26 Jul 2016 02:31
Last Modified: 08 Aug 2016 23:35

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