Differential effects of two-pore channel protein 1 and 2 silencing in MDA-MB-468 breast cancer cells

Jahidin, Aisyah H., Stewart, Teneale A., Thompson, Erik W., Roberts-Thomson, Sarah J., & Monteith, Gregory R. (2016) Differential effects of two-pore channel protein 1 and 2 silencing in MDA-MB-468 breast cancer cells. Biochemical and Biophysical Research Communications, 477(4), pp. 731-736.

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Abstract

Two-pore channel proteins, TPC1 and TPC2, are calcium permeable ion channels found localized to the membranes of endolysosomal calcium stores. There is increasing interest in the role of TPC-mediated intracellular signaling in various pathologies; however their role in breast cancer has not been extensively evaluated. TPC1 and TPC2 mRNA was present in all non-tumorigenic and tumorigenic breast cell lines assessed. Silencing of TPC2 but not TPC1 attenuated epidermal growth factor-induced vimentin expression in MDA-MB-468 breast cancer cells. This effect was not due to a general inhibition of epithelial to mesenchymal transition (EMT) as TPC2 silencing had no effect on epidermal growth factor (EGF)-induced changes on E-cadherin expression. TPC1 and TPC2 were also shown to differentially regulate cyclopiazonic acid (CPA)-mediated changes in cytosolic free Ca2+. These findings indicate potential differential regulation of signaling processes by TPC1 and TPC2 in breast cancer cells. © 2016 Elsevier Inc.

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ID Code: 97708
Item Type: Journal Article
Refereed: Yes
Keywords: Breast cancer, Calcium, Calcium signaling, Epidermal growth factor, Two-pore channel, Vimentin
DOI: 10.1016/j.bbrc.2016.06.127
ISSN: 0006-291X
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Cell Biology (111201)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2016 Elsevier Inc.
Deposited On: 29 Jul 2016 04:13
Last Modified: 31 Jul 2016 22:10

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