Isolation and expansion of placental derived mesenchymal stromal cells in a packed bed bioreactor

Osiecki, Michael J. (2016) Isolation and expansion of placental derived mesenchymal stromal cells in a packed bed bioreactor. PhD thesis, Queensland University of Technology.


Mesenchymal stromal cells (MSCs) are thought to be useful in wound healing applications, but are not available from human sources in large enough quantities to be clinically viable. To isolate and expand placental derived human MSCs, this project details the design and scale up of a novel packed bed bioreactor that decouples the oxygen supply from the bulk medium flow by allowing oxygen to diffuse through the reactor wall. The design was further optimised through finite element modelling to expand up to 3x10^8 MSCs. In addition, modelling provided insight into the reduced cellular growth rate that was observed in the bioreactor compared to traditional tissue culture methods, highlighting the importance of the lactate removal and initial cell distribution on cell growth rate.

Impact and interest:

1 citations in Web of Science®
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39 since deposited on 22 Aug 2016
39 in the past twelve months

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ID Code: 97947
Item Type: QUT Thesis (PhD)
Supervisor: Lott, Bill & Kiriakous, Emad
Keywords: Bioreactor, Packed / fixed bed bioreactor, Mesenchymal stromal cell, cell culture expansion, Stem cell isolation, Finite element modelling, Plasma surface modification, conditioned medium, Mass transport, COMSOL
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Institution: Queensland University of Technology
Deposited On: 22 Aug 2016 03:04
Last Modified: 22 Aug 2016 03:04

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