Cerebral microcirculation during mild head injury after a contusion and acceleration experimental model in sheep

Bellapart, Judith, Abi-Fares, Catherine, Cuthbertson, Kylie, Dunster, Kimble, Diab, Sara, Platts, David G., Raffel, Christopher, Gabrielian, Levon, Barnett, Adrian, Paratz, Jennifer, Boots, Rob, & Fraser, John F. (2016) Cerebral microcirculation during mild head injury after a contusion and acceleration experimental model in sheep. Brain Injury. (In Press)

[img] PDF (221kB)
Administrators only | Request a copy from author

View at publisher


Background: Cerebral microcirculation after head injury is heterogeneous and temporally variable. Regions at risk of infarction such as peri-contusional areas are vulnerable to anaemia. However, direct quantification of the cerebral microcirculation is clinically not feasible. This study describes a novel experimental head injury model correlating cerebral microcirculation with histopathology analysis.

Objective: To test the hypothesis that cerebral microcirculation at the ischaemic penumbrae is reduced over time when compared with non-injured regions.

Methods: Merino sheep were instrumented using a transeptal catheter to inject coded microspheres into the left cardiac atrium, ensuring systemic distribution. After a blunt impact over the left parietal region, cytometric analyses quantified cerebral microcirculation and amyloid precursor protein staining identified axonal injury in pre-defined anatomical regions. A mixed effect regression model assessed the hourly blood flow results during 4 hours after injury.

Results: Cerebral microcirculation showed temporal reductions with minimal amyloid staining except for the ipsilateral thalamus and medulla.

Conclusion: The spatial heterogeneity and temporal reduction of cerebral microcirculation in ovine models occur early, even after mild head injury, independent of the intracranial pressure and the level of haemoglobin. Alternate approaches to ensure recovery of regions with reversible injury require a targeted assessment of cerebral microcirculation.

Impact and interest:

0 citations in Scopus
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 98448
Item Type: Journal Article
Refereed: Yes
Keywords: intensive care, brain injury, microcirculation
DOI: 10.1080/02699052.2016.1199894
ISSN: 1362-301X
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > Schools > School of Public Health & Social Work
Copyright Owner: Copyright 2016 Taylor & Francis Group, LLC
Copyright Statement: The Version of Record of this manuscript has been published and is available in Brain Injury, 26 August 2016, http://www.tandfonline.com/10.1080/02699052.2016.1199894
Deposited On: 29 Aug 2016 01:02
Last Modified: 06 Sep 2016 17:18

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page