An improved 15N tracer approach to study denitrification and nitrogen turnover in soil incubations
Scheer, Clemens, Meier, Rudolf, Brüggemann, Nicolas, Grace, Peter R., & Dannenmann, Michael (2016) An improved 15N tracer approach to study denitrification and nitrogen turnover in soil incubations. Rapid Communications in Mass Spectrometry, 30(18), pp. 2017-2026.
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Rationale: Denitrification (the reduction of oxidized forms of inorganic N to N2O and N2) from upland soils is considered to be the least well understood process in the global N cycleDenitrification from upland soils is considered to be the least well understood process in the global N cycle due to methodical constraints of existing methods. . The main reason for this lack of understanding is that the terminal product (N2) of denitrification is extremely difficult to measure against the hugelarge atmospheric background.
Methods: Here wWe describe a system that combines the 15N-tracer technique with a 40-fold reduced N2 (2 % v/v) atmosphere in a fully automated incubation set up for direct quantification of N2 and N2O emissions. The system was tested on a pasture soil from sub-tropical Australia under different soil moisture conditions and combined with 15N tracing in extractable soil N pools to establish a full N balance.
Results: The method proved to be highly sensitive for detecting N2 (1.12 µg N h-1 kg-1ds1 dry soil (ds)) and N2O (0.36 µg N h-1 kg 1 ds) emissions. The main end-product of denitrification in the investigated soil was N2O for both water contents with N2 accounting for only 3% to13% of the total denitrification losses. Between 90-95% of the added 15N fertiliser could be recovered in N gases and extractable soil N pools.
Conclusions: These high and N2O-dominated denitrification rates found in this study are pointing at both a high ecological and agronomic importance of denitrification in subtropical pasture soils. The new system allows for a direct and highly sensitive detection of N2 and N2O fluxes from soils and may help to significantly improve our mechanistic understanding of N cycling and denitrification in terrestrial agro-ecosystems.
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|Item Type:||Journal Article|
|Divisions:||Current > Schools > School of Earth, Environmental & Biological Sciences
Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute for Future Environments
|Copyright Owner:||Copyright 2016 John Wiley & Sons, Ltd.|
|Copyright Statement:||This is the peer reviewed version of the following article: An improved 15N tracer approach to study denitrification and nitrogen turnover in soil incubations, Rapid Communications in Mass Spectrometry, Volume 30, Issue 18, pages 2017–2026, 30 September 2016, which has been published in final form at https://doi.org/10.1002/rcm.7689. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.|
|Deposited On:||07 Sep 2016 00:45|
|Last Modified:||13 Sep 2016 18:19|
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