A pilot study to demonstrate diagnostic potential of galectin-3 levels in saliva

Zhang, Xi, Wan, Yunxia, Chata, Roberto, Brazzale, Anthony, Atherton, John J., Kostner, Karam, Dimeski, Goce, & Punyadeera, Chamindie (2016) A pilot study to demonstrate diagnostic potential of galectin-3 levels in saliva. Journal of Clinical Pathology. (In Press)

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Abstract

Aim

  • Heart failure (HF) affects millions of older individuals in both developed and low/middle-income countries. Serum galectin-3 levels have been shown to have prognostic value. However, its use as a diagnostic biomarker has not been explored. The aim was to establish a saliva galectin-3 reference range and to demonstrate the potential diagnostic utility of salivary and serum galectin-3 levels in assessing HF.

Methods

  • Blood and saliva samples were collected from age-matched healthy controls (n=51) and patients with HF (n=63). Customised immunoassays were developed to quantify salivary galectin-3 levels. The diagnostic performances of these assays were evaluated by receiver operator characteristic (ROC) curves analysis.

Results

  • The galectin-3 concentrations were significantly elevated in saliva and serum samples of patients with HF compared with controls (p<0.001 and p<0.0001, respectively). Using ROC curve analysis, both serum and salivary galectin-3 gave area under the curve (AUC)=0.86 and AUC=0.73, respectively. There was also a significant correlation (r=0.4, p<0.01) between serum and salivary galectin-3 levels.

Conclusions

  • For the first time, we have quantified galectin-3 levels in human saliva and have demonstrated potential clinical utility in diagnosing HF. Further, larger multicentre clinical trials are needed before salivary galectin-3 levels can be implemented in a clinical setting.

Impact and interest:

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ID Code: 98877
Item Type: Journal Article
Refereed: Yes
Keywords: Cardiovascular disease, Serum galectin-3, prognostic, diagnostic potential, saliva, heart failure, blood, Saliva galectin-3
DOI: 10.1136/jclinpath-2016-203631
ISSN: 1472-4146
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > CARDIOVASCULAR MEDICINE AND HAEMATOLOGY (110200) > Cardiology (incl. Cardiovascular Diseases) (110201)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > CARDIOVASCULAR MEDICINE AND HAEMATOLOGY (110200) > Respiratory Diseases (110203)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > CARDIOVASCULAR MEDICINE AND HAEMATOLOGY (110200) > Cardiovascular Medicine and Haematology not elsewhere classified (110299)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: author (or their employer) 2016. Produced by BMJ Publishing Group Ltd under licence.
Deposited On: 15 Sep 2016 23:36
Last Modified: 20 Sep 2016 01:36

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