Direct synthesis of well-defined heterotelechelic polymers for bioconjugations

Boyer, C., Liu, J., Bulmus, V., Davis, T. P., Barner-Kowollik, C., & Stenzel, M. H. (2008) Direct synthesis of well-defined heterotelechelic polymers for bioconjugations. Macromolecules, 41(15).

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Abstract

Direct synthesis of well-defined heterotelechelic polymers having functional groups allowing the chemoselective bioconjugations would be desirable to enhance the versatility of polymers for bioconjugations and bio-related applications. Considering this, well-defined α-azide, ω- dithiopyridine polymers were synthesized in one step via the reversible addition-fragmentation chain transfer (RAFT) polymerization. The telechelic functionality (i.e., mole ratio of the ω-dithiopyridine to the ̌-azide end group) of polymers was above 0.90, indicating the efficient generation of well-defined heterotelechelic polymers. The heterotelechelic functionality for chemoselective bioconjugations was tested by reacting α-azide, ω-dithiopyridine poly(NIPAAm) with model biomolecules, i.e., biotin/avidin, glutathione, and bovine serum albumin, via click and thiol-disulfide exchange chemistries. Near-stoichiometric conjugation with biomolecules indicated high functionality of the polymer end groups. © 2008 American Chemical Society.

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ID Code: 99172
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By :111
Export Date: 5 September 2016
CODEN: MAMOB
Correspondence Address: Bulmus, V.; Centre for Advanced Macromolecular Design (CAMD), School of Chemical Sciences and Engineering, University of New South Wales (UNSW), Sydney, NSW 2052, Australia; email: vbulmus@unsw.edu.au
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Keywords: Biomolecules, Body fluids, Chemical reactions, Functional groups, Functional polymers, Molecular biology, Monomers, Photoresists, Sulfur compounds, Synthesis (chemical), Applications., Bioconjugations, Bovine serum albumin, Chemo-selective, Direct synthesis, End groups, Glutathione, Mole ratios, One step, Reversible addition- fragmentation chain transfer, Telechelic, Thiol-disulfide, Polymers
DOI: 10.1021/ma800289u
ISSN: 00249297
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 22 Sep 2016 04:50
Last Modified: 10 Oct 2016 02:01

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