Apical cell surface expression of rat dipeptidyl peptidase IV in transfected madin-darby canine kidney cells

Low, Seng Hui, Wong, Siew Heng, Tang, Bor Luen, Subramaniam, V. Nathan, & Hong, Wanjin (1991) Apical cell surface expression of rat dipeptidyl peptidase IV in transfected madin-darby canine kidney cells. Journal of Biological Chemistry, 266(20), pp. 13391-13396.

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Abstract

Dipeptidyl peptidase IV (DPPIV) is a type II membrane glycoprotein that is predominantly localized to the apical plasma membrane in various epithelial cells. In order to understand in more detail the biogenesis and sorting of DPPIV, the cDNA for rat DPPIV was inserted into a mammalian plasmid expression vector so that DPPIV expression was driven by a control region composed of the SV40 early promoter region fused to the enhancer of the Rous sarcoma virus. Madin-Darby canine kidney cells transfected with this construct were found to express the DPPIV protein. In these transfected cells, the majority of DPPIV was present on the apical cell surface. This observation suggests that the information for apical surface localization is inherent in the DPPIV molecule itself and that this sorting information is decipherable in the epithelial cells of a different species. DPPIV is transported efficiently from the endoplasmic reticulum to the Golgi apparatus as assessed by pulse-chase experiments. Furthermore, evidence is presented which suggests that the majority of DPPIV is sorted intracellularly to the apical cell surface. The same protein has, however, been reported to be sorted by an indirect pathway through transcytosis from the basolateral to the apical cell surface in hepatocytes (Bartles, J. R., Feracci, H., M., Stinger, B., and Hubbard, A. L. (1987) J. Cell Biol. 105, 1241-1251). This study suggests that the same protein can take two different pathways in different cell types for its correct apical cell surface localization.

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52 citations in Web of Science®
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ID Code: 99902
Item Type: Journal Article
Refereed: Yes
Additional Information: This research was originally published in the Journal of Biological Chemistry. S H Low, S H Wong, B L Tang, V N Subramaniam and W J Hong. Apical cell surface expression of rat dipeptidyl peptidase IV in transfected Madin-Darby canine kidney cells. Journal of Biological Chemistry. 1991; 266:13391-13396. © the American Society for Biochemistry and Molecular Biology.
ISSN: 1083-351X
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: Copyright 1991 The American Society for Biochemistry and Molecular Biology, Inc.
Deposited On: 13 Oct 2016 05:44
Last Modified: 28 Jun 2017 20:02

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