Inhibition by brefeldin A of protein secretion from the apical cell surface of Madin-Darby canine kidney cells

Low, Seng Hui, Wong, Siew Heng, Tang, Bor Luen, Tan, Patrick, Subramaniam, V. Nathan, & Hong, Wanjin (1991) Inhibition by brefeldin A of protein secretion from the apical cell surface of Madin-Darby canine kidney cells. The Journal of Biological Chemistry, 266(27), pp. 17729-17732.

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The effect of brefeldin A (BFA) on total and polarized protein secretion was examined in MDCK cells. Increasing concentrations of BFA have increasingly inhibitory effects on total protein secretion. The total protein secretion was essentially unaffected by BFA at 0.5 μg/ml. When the BFA concentration was increased to 10 and 30 μg/ml, the total protein secretion was reduced to about 70 and 25, respectively, of the control level. Consistent with this effect on total protein secretion, the Golgi structure as revealed by C6-NBD-ceramide (a fluorescent ceramide analog) staining was essentially unaltered by 0.5 μg/ml BFA, while 10 and 30 μg/ml BFA significantly dispersed the Golgi apparatus. When the polarity of protein secretion was examined, it was found that the ratio of proteins secreted from the apical to those from the basolateral surface was reduced from 1.5-2.0 to 0.4-0.7 by all three BFA concentrations. Furthermore, several proteins which are preferentially released from the apical surface were found to be released without apparent surface polarity, while several other proteins which were preferentially released from the basolateral surface were unaffected. This study suggests that BFA, at 0.5 μg/ml, can selectively inhibit protein secretion from the apical surface without affecting total protein secretion. The inhibition of apical secretion results in enhanced protein secretion from the basolateral surface.

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60 citations in Web of Science®
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ID Code: 99903
Item Type: Journal Article
Refereed: Yes
ISSN: 1083-351X
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: Copyright 1991 The American Society for Biochemistry and Molecular Biology, Inc.
Deposited On: 13 Oct 2016 05:52
Last Modified: 17 May 2017 04:58

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