Development of a redox-responsive polymeric profluorescent probe

Hansen, Kai-Anders, Fairfull-Smith, Kathryn E., Bottle, Steven E., & Blinco, James P. (2016) Development of a redox-responsive polymeric profluorescent probe. Macromolecular Chemistry and Physics. (In Press)

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Profluorescent nitroxides (PFNs) have emerged as an important class of imaging agents for monitoring intracellular redox status and levels of oxidative stress. However, the fast reduction of nitroxides upon incubation within cells limits the window of opportunity for detection. By increasing the concentration of nitroxides per fluorophore, their reduction to the corresponding hydroxylamines and the subsequent switch-on of fluorescence can be delayed. Herein the preparation of nitroxide-containing polymers of different chain length coupled to a fluorophore is reported and their reduction with pentafluorophenylhydrazine is examined. The fluorescence switch-on kinetics and radical concentrations are monitored by fluorescence and electron paramagnetic resonance spectroscopy and compared to a conventional PFN bearing a single nitroxide moiety. The polymeric PFNs display significant delays in reduction and fluorescence switch-on and higher turn-on ratios than their single-nitroxide counterparts. The results of this study indicate that polymeric PFNs are a promising architecture for future imaging agents.

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ID Code: 99915
Item Type: Journal Article
Refereed: Yes
DOI: 10.1002/macp.201600147
ISSN: 1022-1352
Divisions: Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Deposited On: 12 Oct 2016 01:02
Last Modified: 13 Oct 2016 05:20

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