Overcoming instability of antibody-nanomaterial conjugates: Next generation targeted nanomedicines using bispecific antibodies

Howard, Christopher B., Fletcher, Nicholas, Houston, Zachary H., Fuchs, Adrian V., Boase, Nathan R.B., Simpson, Joshua D., Raftery, Lyndon J., Ruder, Tim, Jones, Martina L., de Bakker, Christopher J., Mahler, Stephen M., & Thurecht, Kristofer J. (2016) Overcoming instability of antibody-nanomaterial conjugates: Next generation targeted nanomedicines using bispecific antibodies. Advanced Healthcare Materials, 5(16), pp. 2055-2068.

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Targeted nanomaterials promise improved therapeutic efficacy, however their application in nanomedicine is limited due to complexities associated with protein conjugations to synthetic nanocarriers. A facile method to generate actively targeted nanomaterials is developed and exemplified using polyethylene glycol (PEG)-functional nanostructures coupled to a bispecific antibody (BsAb) with dual specificity for methoxy PEG (mPEG) epitopes and cancer targets such as epidermal growth factor receptor (EGFR). The EGFR-mPEG BsAb binds with high affinity to recombinant EGFR (KD : 1 × 10-9 m) and hyperbranched polymer (HBP) consisting of mPEG (KD : 10 × 10-9 m) and demonstrates higher avidity for HBP compared to linear mPEG. The binding of BsAb-HBP bioconjugate to EGFR on MDA-MB-468 cancer cells is investigated in vitro using a fluorescently labeled polymer, and in in vivo xenograft models by small animal optical imaging. The antibody-targeted nanostructures show improved accumulation in tumor cells compared to non-targeted nanomaterials. This demonstrates a facile approach for tuning targeting ligand density on nanomaterials, by modulating surface functionality. Antibody fragments are tethered to the nanomaterial through simple mixing prior to administration to animals, overcoming the extensive procedures encountered for developing targeted nanomedicines.

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ID Code: 99926
Item Type: Journal Article
Refereed: Yes
Keywords: bispecific antibodies;epidermal growth factor receptor;poly(ethylene glycol);polymers;targeted nanomaterials
DOI: 10.1002/adhm.201600263
ISSN: 21922640
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 12 Oct 2016 23:54
Last Modified: 13 Oct 2016 23:37

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