Unlocking Hidden Genomic Sequence

Keith, Jonathan M. and Cochran, Duncan A. and Lala, Gita H. and Adams, Peter and Bryant, Darryn and Mitchelson, Keith R. (2004) Unlocking Hidden Genomic Sequence. Nucleic Acids Research 32(3):e35.

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Abstract

Despite the success of conventional Sanger sequencing, signi®cant regions of many genomes still present major obstacles to sequencing. Here we propose a novel approach with the potential to alleviate a wide range of sequencing dif®culties. The technique involves extracting target DNA sequence from variants generated by introduction of random mutations. The introduction of mutations does not destroy original sequence information, but distributes it amongst multiple variants. Some of these variants lack problematic features of the target and are more amenable to conventional sequencing. The technique has been successfully demonstrated with mutation levels up to an average 18% base substitution and has been used to read previously intractable poly(A), AT-rich and GC-rich motifs.

Item Type:	Journal Article
Status:	Published
Subjects:	270000 Biological Sciences > 270800 Biotechnology
ID Code:	8016
Deposited By:	Keith, Jonathan M
Deposited On:	06 June 2007
Alternative Locations:	http://dx.doi.org/10.1093/nar/gnh022
Copyright Owner:	Copyright 2004 Oxford University Press
Copyright Statement:	This is an electronic version of an article published in [Nucleic Acids Research 32(3):e35]