SpheroidSim - preliminary evaluation of a new computational tool to predict the influence of cell cycle time and phase fraction on spheroid growth

, , , & (2018) SpheroidSim - preliminary evaluation of a new computational tool to predict the influence of cell cycle time and phase fraction on spheroid growth. Biotechnology Progress, 34(6), pp. 1335-1343.

[img]
Preview
Accepted Version (PDF 1MB)
Manuscript_2018_03_29 ePrints.pdf.

View at publisher

Description

Background: There is a relative paucity of research that integrates materials science and bioengineering with computational simulations to decipher the intricate processes promoting cancer progression. Therefore, a first-generation computational model, SpheroidSim, was developed that includes a biological data set derived from a bioengineered spheroid model to obtain a quantitative description of cell kinetics. Results: SpheroidSim is a 3D agent-based model simulating the growth of multi-cellular cancer spheroids. Cell cycle time and phases mathematically motivated the population growth. SpheroidSim simulated the growth dynamics of multiple spheroids by individually defining a collection of specific phenotypic traits and characteristics for each cell. Experimental data derived from a hydrogel-based spheroid model were fit to the predictions providing insight into the influence of cell cycle time (CCT) and cell phase fraction (CPF) on the cell population. A comparison of the number of active cells predicted for each analysis showed that the value and method used to define CCT had a greater effect on the predicted cell population than CPF. The model predictions were similar to the experimental results for the number of cells, with the predicted total number of cells varying by 8% and 12%, respectively, compared to the experimental data. Conclusions: SpheroidSim is a first step in developing a biologically-based predictive tool capable of revealing fundamental elements in cancer cell physiology. This computational model may be applied to study the effect of the microenvironment on spheroid growth and other cancer cell types that demonstrate a similar multi-cellular clustering behaviour as the population develops.

Impact and interest:

0 citations in Scopus
0 citations in Web of Science®
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

130 since deposited on 10 Sep 2018
9 in the past twelve months

Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 121214
Item Type: Contribution to Journal (Journal Article)
Refereed: Yes
ORCID iD:
Little, Paigeorcid.org/0000-0001-8377-5343
Hutmacher, Dietmarorcid.org/0000-0001-5678-2134
Loessner, Danielaorcid.org/0000-0001-5891-3441
Measurements or Duration: 9 pages
Keywords: agent-based model, bioengineering, cancer spheroids, cell growth, mathematical modelling
DOI: 10.1002/btpr.2692
ISSN: 8756-7938
Pure ID: 33367560
Divisions: Past > Institutes > Institute of Health and Biomedical Innovation
Past > QUT Faculties & Divisions > Science & Engineering Faculty
Copyright Owner: Consult author(s) regarding copyright matters
Copyright Statement: This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au
Deposited On: 10 Sep 2018 03:59
Last Modified: 10 Feb 2025 16:41