Characterisation of the Pseudomonas savastanoi pv. phaseolicola population found in Eastern Australia associated with halo blight disease in Vigna radiata
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Description
This study analysed the phenotypic and genotypic variation among 511 Pseudomonas savastanoi pv. phaseolicola (Psp) isolates, causing halo blight in mungbeans. Collected from symptomatic mungbean (Vigna radiata) crops throughout Australia between 2005 and 2018, a total of 352 Psp isolates were phenotypically screened. Our in planta screening against a set of four mungbean cultivars with known susceptible and resistant reactions revealed five distinctive pathotypes. Isolates belonging to pathotype 2 were the most prevalent at 84% and were found to be highly pathogenic towards all tested mungbean genotypes. Genomic variation was investigated for 205 isolates using DNA fingerprints, splitting the halo blight pathogen population into two broad genetic lineages. Further genetic testing for two known avirulence genes, avrPphE and avrPphF, identified the avrPphE gene in all the tested isolates and avrPphF present in all but two. To identify candidate avirulence genes unique to Psp isolates infecting mungbean in Australia, a comparative genomics study was undertaken on the whole-genome sequences of two epidemiologically important Psp isolates, T11544 and K4287. The information presented in this study has the potential to dramatically improve mungbean disease resistance now and into the future.
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| ID Code: | 202286 | ||||||
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| Item Type: | Contribution to Journal (Journal Article) | ||||||
| Refereed: | Yes | ||||||
| ORCID iD: |
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| Measurements or Duration: | 10 pages | ||||||
| Keywords: | Avirulence, BOX, ERIC, IS50, Mungbean, Pathotype | ||||||
| DOI: | 10.1007/s13313-020-00722-8 | ||||||
| ISSN: | 0815-3191 | ||||||
| Pure ID: | 62980581 | ||||||
| Divisions: | Current > Research Centres > Centre for Agriculture and the Bioeconomy Past > Institutes > Institute for Future Environments Past > QUT Faculties & Divisions > Science & Engineering Faculty Current > QUT Faculties and Divisions > Faculty of Science Current > Schools > School of Biology & Environmental Science Past > QUT Faculties & Divisions > Division of Research and Innovation Current > Research Centres > Centre for Tropical Crops and Biocommodities |
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| Funding Information: | This work was supported by the Australian Mungbean Association PhD scholarship program (2017000885) and the Centre for Tropical Crops and Biocommodities, Queensland University of Technology, Brisbane, Qld. The Grains and Research and Development Corporation (GRDC) and The Department of Agriculture and Fisheries of Queensland, Australia investments in grains pathology project (DAQ00186) and the National Mungbean Improvement Program (DAQ00210). We acknowledge the Office of eResearch at QUT for providing access to compute infrastructure and bioinformatics pipelines for this study. We also thank the industry as a whole for their support in providing samples from across Australia. (Burkholder 1926 ). | ||||||
| Copyright Owner: | The Author(s) 2020 | ||||||
| Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||||||
| Deposited On: | 21 Jul 2020 03:39 | ||||||
| Last Modified: | 10 May 2024 15:01 |
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