Comparative TMT Proteomic Analysis Unveils Unique Insights into Helicoverpa armigera (Hübner) Resistance in Cajanus scarabaeoides (L.) Thouars
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Description
Pigeonpea [Cajanus cajan (L.) Millspaugh] is an economically important legume playing a crucial role in the semi‐arid tropics. Pigeonpea is susceptible to Helicoverpa armigera (Hübner), which causes devastating yield losses. This pest is developing resistance to many commercially available insecticides. Therefore, crop wild relatives of pigeonpea, are being considered as potential sources of genes to expand the genetic base of cultivated pigeonpea to improve traits such as host plant resistance to pests and pathogens. Quantitative proteomic analysis was conducted using the tandem mass tag platform to identify differentially abundant proteins between IBS 3471 and ICPL 87 tolerant accession and susceptible variety to H. armigera, respectively. Leaf proteome were analysed at the vegetative and flowering/podding growth stages. H. armigera tolerance in IBS 3471 appeared to be related to enhanced defence responses, such as changes in secondary metabolite precursors, an-tioxidants, and the phenylpropanoid pathway. The development of larvae fed on an artificial diet with IBS 3471 lyophilised leaves showed similar inhibition with those fed on an artificial diet with quercetin concentrations with 32 mg/25 g of artificial diet. DAB staining (3,3’‐diaminobenzidine) revealed a rapid accumulation of reactive oxygen species in IBS 3471. We conclude that IBS 3471 is an ideal candidate for improving the genetic base of cultivated pigeonpea, including traits for host plant resistance.
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| ID Code: | 211060 | ||||||
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| Item Type: | Contribution to Journal (Journal Article) | ||||||
| Refereed: | Yes | ||||||
| ORCID iD: |
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| Additional Information: | Funding: This project was funded by Advance Queensland Research Fellowship package grant numbers AQRF14816‐17RD2, AQRF04016‐17RD2 and AQRF14716‐17RD2. A.N.‐D. is a QUT‐HDR Sponsorship and Agrocorp scholarship recipient. A.N.‐D. was also supported by QUT Giving Day (2019) ‘Feed the World’ program. | ||||||
| Measurements or Duration: | 20 pages | ||||||
| Keywords: | Cajanus cajan, Feeding bioassays, IBS 3471, ICPL 87, Phenypropanoid pathway, Pigeonpea, Quercetin, TMT | ||||||
| DOI: | 10.3390/ijms22115941 | ||||||
| ISSN: | 1422-0067 | ||||||
| Pure ID: | 86251351 | ||||||
| Divisions: | Current > Research Centres > Centre for Agriculture and the Bioeconomy Current > QUT Faculties and Divisions > Faculty of Science Current > Schools > School of Biology & Environmental Science |
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| Funding Information: | Acknowledgments: The authors thank Hao Long (QUT‐CAB), Chalani Marasinghege (QUT‐CAB), Roberto Barrero (QUT‐CARF), David Marshal (QUT‐ CARF), Raj Gupta (QUT‐ CARF), and Pawel Sadowski (QUT‐CARF) for providing technical support. We thank The Australian Grains Genebank (AGG), (Horsham, Victoria, Australia) for providing seeds used in the study. This research has been facilitated by access to Macquarie University’s Australian Proteome Analysis Facility, which is funded by an initiative of the Australian Government, as part of the National Collaborative Research Infrastructure Strategy. This project was funded by Advance Queensland Research Fellowship package grant numbers AQRF14816?17RD2, AQRF04016?17RD2 and AQRF14716?17RD2. A.N.?D. is a QUT?HDR Sponsorship and Agrocorp scholarship recipient. A.N.?D. was also supported by QUT Giving Day (2019) ?Feed the World? program.The authors thank Hao Long (QUT?CAB), Chalani Marasinghege (QUT?CAB), Roberto Barrero (QUT?CARF), David Marshal (QUT? CARF), Raj Gupta (QUT? CARF), and Pawel Sadowski (QUT?CARF) for providing technical support. We thank The Australian Grains Genebank (AGG), (Horsham, Victoria, Australia) for providing seeds used in the study. This research has been facilitated by access to Macquarie University?s Australian Proteome Analysis Facility, which is funded by an initiative of the Australian Government, as part of the National Collaborative Research Infrastructure Strategy. Funding: This project was funded by Advance Queensland Research Fellowship package grant numbers AQRF14816‐17RD2, AQRF04016‐17RD2 and AQRF14716‐17RD2. A.N.‐D. is a QUT‐HDR Sponsorship and Agrocorp scholarship recipient. A.N.‐D. was also supported by QUT Giving Day (2019) ‘Feed the World’ program. | ||||||
| Copyright Owner: | 2021 The Author(s) | ||||||
| Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||||||
| Deposited On: | 14 Jun 2021 02:57 | ||||||
| Last Modified: | 17 May 2024 18:42 |
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