A Longitudinal Case Series Investigating Cellular Changes to the Transplanted Cornea Using Confocal Microscopy

PURPOSE: To perform a longitudinal evaluation of subjects who had undergone penetrating keratoplasty, using slit scanning confocal microscopy. METHODS: In vivo confocal microscopy was used to evaluate the central cornea of four subjects who had recently undergone penetrating keratoplasty. Subjects were examined on four occasions over a 12-month period after surgery. Quantitative and qualitative aspects of corneal morphology were compared against data from normal control subjects. RESULTS: The epithelium varied in appearance between subjects and took at least 12 months to return to a similar arrangement to that seen in normal eyes. Bowman's layer was viewed as an acellular layer immediately after surgery with no evidence of nerve fibres, although nerve components were apparent 12 months after surgery. Stromal nerves were not visible immediately after surgery. One year following penetrating keratoplasty there was evidence of thin nerves running a straight course through the central stroma. Keratocyte density in the anterior and posterior stroma was lower in the transplanted cornea but appeared to remain constant over a period of 1 year. Activated keratocytes were seen in the anterior stroma of all subjects; they appeared to be responsible for significant levels of corneal haze. The time period within which this keratocyte activation occurred varied between individuals. Endothelial cell density decreased at an accelerated rate over the 12-month period. CONCLUSIONS: Confocal microscopy allows cellular changes to be monitored in vivo following penetrating keratoplasty and may assist clinicians in understanding postoperative recovery.